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Mouse Anti-AHCY Recombinant Antibody (V2-364473) (CBMAB-S1397-CQ)

This product is a mouse antibody that recognizes AHCY. The antibody CBXS-4144 can be used for immunoassay techniques such as: ELISA, WB, IHC, IF.
See all AHCY antibodies
Published Data
Mouse Anti-AHCY Recombinant Antibody (CBXS-4144) (CBMAB-S1397-CQ) in IP
Immunochemical analysis with the indicated antibodies of lysates from CD133high cells in which CD133 expression was down-modulated. Tubulin was blotted as a loading control. The data are representative of three separate experiments. ...View More
Brugnoli, F., Grassilli, S., Piazzi, M., Palomba, M., Nika, E., Bavelloni, A., ... & Bertagnolo, V. (2013). In triple negative breast tumor cells, PLC-β2 promotes the conversion of CD133 high to CD133 low phenotype and reduces the CD133-related invasiveness. Molecular cancer, 12, 1-15.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-364473
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IP, IF

Basic Information

Immunogen
Amino acids 19-47 near the N-terminus of SAHH of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adenosylhomocysteinase
Introduction
S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
191
UniProt ID
P23526
Alternative Names
Adenosylhomocysteinase; S-Adenosyl-L-Homocysteine Hydrolase; S-Adenosylhomocysteine Hydrolase; EC 3.3.1.1; AdoHcyase; SAHH;
Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Biological Process
Chronic inflammatory response to antigenic stimulus
Circadian sleep/wake cycle
Methylation
One-carbon metabolic process
Response to hypoxia
Response to nutrient
S-adenosylhomocysteine catabolic process
S-adenosylmethionine cycle
Sulfur amino acid metabolic process
Cellular Location
Cytoplasm; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Involvement in disease
Hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD): A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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