Human AHCY ELISA Kit (V2LY-0626-LY4321)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
47.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Adenosylhomocysteinase
Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Biological Process
Chronic inflammatory response to antigenic stimulus
Circadian sleep/wake cycle
Methylation
One-carbon metabolic process
Response to hypoxia
Response to nutrient
S-adenosylhomocysteine catabolic process
S-adenosylmethionine cycle
Sulfur amino acid metabolic process
Cellular Location
Cytoplasm; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Involvement in disease
Hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD): A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.

Gupta, A., & Storey, K. B. (2021). Coordinated expression of Jumonji and AHCY under OCT transcription factor control to regulate gene methylation in wood frogs during anoxia. Gene, 788, 145671.

Vizán, P., Di Croce, L., & Aranda, S. (2021). Functional and pathological roles of AHCY. Frontiers in Cell and Developmental Biology, 9, 587.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

AHCY Matched Antibody Pair (033) (CAT#: APMAB-033LY)

Antibody Pairs

AHCY Matched Antibody Pair (033) (CAT#: APMAB-033LY)

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Isotype control

For research use only. Not intended for any clinical use.

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