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Mouse Anti-AHNAK Recombinant Antibody (V2-12541) (CBMAB-1031-CN)

This product is a mouse antibody that recognizes AHNAK of human. The antibody 2014C3a can be used for immunoassay techniques such as: WB.
See all AHNAK antibodies
Published Data
Mouse Anti-AHNAK Recombinant Antibody (1G11) (CBMAB-1031-CN) in WB
AHNAK was upregulated in stenotic tracts of HSCR patients at mRNA and protein level compared with control tracts. ...View More
Zhou, L., Zhi, Z., Chen, P., Du, C., Wang, B., Fang, X., ... & Li, H. (2022). LncRNA-RMST Functions as a Transcriptional Co-regulator of SOX2 to Regulate miR-1251 in the Progression of Hirschsprung's Disease. Frontiers in Pediatrics, 10.
Mouse Anti-AHNAK Recombinant Antibody (1G11) (CBMAB-1031-CN) in ICC
Expression and subcellular location of endogenous CAP and AHNAK. Endogenous CAP and AHNAK were observed by confocal microscopy. CAP and AHNAK were located in the cytoplasm in the HCT-116 control group. CAP and AHNAK were simultaneously located in not only the cytoplasm but also in the nucleus. Nucleus (blue); CAP (green); AHNAK (red). AHNAK, AHNAK nucleoprotein; CAP, Cbl-associated protein. ...View More
Cho, W. C., Jang, J. E., Kim, K. H., Yoo, B. C., & Ku, J. L. (2020). SORBS1 serves a metastatic role via suppression of AHNAK in colorectal cancer cell lines. International journal of oncology, 56(5), 1140-1151.
Mouse Anti-AHNAK Recombinant Antibody (1G11) (CBMAB-1031-CN) in WB
Protein expression of cancer metastasis-associated proteins in response to SORBS1 knockdown. Protein expression was detected by western blotting. Cells were treated with 1 mM metformin and the expression levels of SORBS1-related proteins were measured after 2 h. (A) Whole cell lysates, and (B) cytoplasmic and nuclear protein fractions were assessed. AHNAK, AHNAK nucleoprotein; p, phosphorylated; ROCK1, Rho-associated coiled-coil containing protein kinase 1; sh, short hairpin RNA; SORBS1, sorbin and SH3 domain-containing; 1 t, total. ...View More
Cho, W. C., Jang, J. E., Kim, K. H., Yoo, B. C., & Ku, J. L. (2020). SORBS1 serves a metastatic role via suppression of AHNAK in colorectal cancer cell lines. International journal of oncology, 56(5), 1140-1151.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-12541
Antibody Isotype
IgG2a, κ
Application
WB, IP, ELISA

Basic Information

Immunogen
Recombinant AHNAK protein of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AHNAK Nucleoprotein
Introduction
The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. The protein may be required for neuronal cell differentiation.
Entrez Gene ID
79026
UniProt ID
Q09666
Alternative Names
PM227; AHNAKRS
Function
May be required for neuronal cell differentiation.
Biological Process
Regulation of RNA splicing
Regulation of voltage-gated calcium channel activity
Cellular Location
Nucleus

Kim, Y. N., Shin, J. H., Kyeong, D. S., Cho, S. Y., Kim, M. Y., Lim, H. J., ... & Seong, J. K. (2021). Ahnak deficiency attenuates high-fat diet-induced fatty liver in mice through FGF21 induction. Experimental & molecular medicine, 53(3), 468-482.

Cho, W. C., Jang, J. E., Kim, K. H., Yoo, B. C., & Ku, J. L. (2020). SORBS1 serves a metastatic role via suppression of AHNAK in colorectal cancer cell lines. International journal of oncology, 56(5), 1140-1151.

Zhang, Z., Liu, X., Huang, R., Liu, X., Liang, Z., & Liu, T. (2019). Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition. Journal of Cancer, 10(16), 3860.

Lee, H., Kim, K., Woo, J., Park, J., Kim, H., Lee, K. E., ... & Ryu, H. S. (2018). Quantitative proteomic analysis identifies AHNAK (neuroblast differentiation-associated protein AHNAK) as a novel candidate biomarker for bladder urothelial carcinoma diagnosis by liquid-based cytology. Molecular & Cellular Proteomics, 17(9), 1788-1802.

Zhao, Z., Xiao, S., Yuan, X., Yuan, J., Zhang, C., Li, H., ... & Liu, Q. (2017). AHNAK as a prognosis factor suppresses the tumor progression in glioma. Journal of Cancer, 8(15), 2924.

Chen, B., Wang, J., Dai, D., Zhou, Q., Guo, X., Tian, Z., ... & Xie, X. (2017). AHNAK suppresses tumour proliferation and invasion by targeting multiple pathways in triple-negative breast cancer. Journal of Experimental & Clinical Cancer Research, 36(1), 1-11.

Cho, W. C., Jang, J. E., & Ku, J. L. (2017). SORBS1-AHNAK complex regulates metastatic properties of cancer.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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