Mouse Anti-AHNAK Recombinant Antibody (V2-12542) (CBMAB-1032-CN)

Name: Mouse Anti-AHNAK Recombinant Antibody (V2-12542)
SKU: CBMAB-1032-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AHNAK Recombinant Antibody (EM-09) (CBMAB-1032-CN) in IF

Gm40600 Tg up-regulated the cytoplasmic lateral location of Ahnak. At 48 hrs after the stimulation, the CD4+ T cells were stained intracellularly with FITC-conjugated anti-mouse Ahnak antibodies and DAPI and were imaged and analysed on a GE IN Cell Analyzer 2000. ...View More

He, Y., Fang, Y., Zhai, B., Liu, X., Zhu, G., Zhou, S., ... & Wang, R. (2021). Gm40600 promotes CD4+ T-cell responses by interacting with Ahnak. Immunology, 164(1), 190-206.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-12542

Application

WB, ICC, IP

Immunogen

Bacterially expressed fragment of N-terminal domain of human AHNAK1

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:2,000
IP	1:10-1:500
IHC-F	1:10-1:500
IHC	1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

15mM sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

AHNAK Nucleoprotein

Introduction

The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. The protein may be required for neuronal cell differentiation.

Entrez Gene ID

Human	79026
Mouse	66395

UniProt ID

Human	Q09666
Mouse	E9Q616

Alternative Names

PM227; AHNAKRS

Function

May be required for neuronal cell differentiation.

Biological Process

Regulation of RNA splicing
Regulation of voltage-gated calcium channel activity

Cellular Location

Nucleus

Kim, Y. N., Shin, J. H., Kyeong, D. S., Cho, S. Y., Kim, M. Y., Lim, H. J., ... & Seong, J. K. (2021). Ahnak deficiency attenuates high-fat diet-induced fatty liver in mice through FGF21 induction. Experimental & molecular medicine, 53(3), 468-482.

Cho, W. C., Jang, J. E., Kim, K. H., Yoo, B. C., & Ku, J. L. (2020). SORBS1 serves a metastatic role via suppression of AHNAK in colorectal cancer cell lines. International journal of oncology, 56(5), 1140-1151.

Zhang, Z., Liu, X., Huang, R., Liu, X., Liang, Z., & Liu, T. (2019). Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition. Journal of Cancer, 10(16), 3860.

Lee, H., Kim, K., Woo, J., Park, J., Kim, H., Lee, K. E., ... & Ryu, H. S. (2018). Quantitative proteomic analysis identifies AHNAK (neuroblast differentiation-associated protein AHNAK) as a novel candidate biomarker for bladder urothelial carcinoma diagnosis by liquid-based cytology. Molecular & Cellular Proteomics, 17(9), 1788-1802.

Zhao, Z., Xiao, S., Yuan, X., Yuan, J., Zhang, C., Li, H., ... & Liu, Q. (2017). AHNAK as a prognosis factor suppresses the tumor progression in glioma. Journal of Cancer, 8(15), 2924.

Chen, B., Wang, J., Dai, D., Zhou, Q., Guo, X., Tian, Z., ... & Xie, X. (2017). AHNAK suppresses tumour proliferation and invasion by targeting multiple pathways in triple-negative breast cancer. Journal of Experimental & Clinical Cancer Research, 36(1), 1-11.

Cho, W. C., Jang, J. E., & Ku, J. L. (2017). SORBS1-AHNAK complex regulates metastatic properties of cancer.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

AHNAK Matched Antibody Pair (035) (CAT#: APMAB-035LY)

AHNAK Matched Antibody Pair (036) (CAT#: APMAB-036LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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