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Mouse Anti-AKAP12 Recombinant Antibody (V2-12555) (CBMAB-1045-CN)

This product is a mouse antibody that recognizes AKAP12 of human. The antibody JP74 can be used for immunoassay techniques such as: WB, IP, ICC.
See all AKAP12 antibodies
Published Data
Fig.1 immunoprecipitations (IP) and immunoblot analyses with JP74, JP98, and polyclonal gravin antiserum.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-12555
Antibody Isotype
IgG2a
Application
WB, IP, ICC

Basic Information

Immunogen
Tissue/cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.02-0.04 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
15mM sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
A-Kinase Anchoring Protein 12
Introduction
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. This is an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Entrez Gene ID
Human9590
Mouse83397
Rat83425
UniProt ID
HumanQ02952
MouseQ9WTQ5
RatQ5QD51
Alternative Names
SSeCKS; AKAP250
Function
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Cellular response to interleukin-1 Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
G protein-coupled receptor signaling pathway Source: ProtInc
Hepatic stellate cell activation Source: Ensembl
Modulation of chemical synaptic transmission Source: Ensembl
Negative regulation of vascular permeability Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Positive regulation of hepatic stellate cell migration Source: Ensembl
Positive regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
Positive regulation of oligodendrocyte apoptotic process Source: Ensembl
Positive regulation of protein kinase A signaling Source: InterPro
Regulation of protein kinase A signaling Source: UniProtKB
Regulation of protein kinase C signaling Source: InterPro
Response to electrical stimulus Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Cellular Location
Cytoskeleton; Cell cortex; Membrane. May be part of the cortical cytoskeleton.

Bai, Y., Yuan, R., Luo, Y., Kang, Z., Zhu, H., Qu, L., ... & Song, X. (2021). Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits. Animals, 11(7), 2090.

Mamoor, S. (2021). Differential expression of A-kinase anchoring protein 12 in cancers of the breast.

Fonseca-Camarillo, G., Furuzawa-Carballeda, J., Priego-Ranero, Á. A., Zúñiga, R. B., Martínez-Benítez, B., & Yamamoto-Furusho, J. K. (2021). AKAP12/Gravin is over-expressed in patients with ulcerative colitis. Immunologic Research, 1-7.

Çiçek, H., Saygılı, Ö., Sever, Ö. N., Kaya, V., Ulusal, H., & Yıldırım, M. (2019). The diagnostic role of A-kinase anchoring protein 12, Bcl-2 and high mobility group box Protein-1 levels in breast cancer. Journal of Oncological Sciences, 5(3), 90-95.

Ibarrola Ulzurrun, J. F., Sádaba Sagredo, R., Martínez Martínez, E., García Peña, A., & Gaínza Calleja, A. (2018). Aldosterone impairs mitochondrial function in human cardiac fibroblasts via A-kinase anchor protein 12. Scientific Reports, 2018, 8, 6801.

Lee, H. S., Choi, J., Son, T., Lee, E. J., Kim, J. G., Ryu, S. H., ... & Kim, K. W. (2018). A‑kinase anchoring protein 12 is downregulated in human hepatocellular carcinoma and its deficiency in mice aggravates thioacetamide‑induced liver injury. Oncology letters, 16(5), 5907-5915.

Ibarrola, J., Sadaba, R., Martinez-Martinez, E., Garcia-Peña, A., Arrieta, V., Alvarez, V., ... & Lopez-Andres, N. (2018). Aldosterone impairs mitochondrial function in human cardiac fibroblasts via A-kinase anchor protein 12. Scientific reports, 8(1), 1-11.

Selvaraju, V., Suresh, S. C., Thirunavukkarasu, M., Mannu, J., Foye, J. L., Mathur, P. P., ... & Maulik, N. (2017). Regulation of A-kinase-anchoring protein 12 by heat shock protein A12B to prevent ventricular dysfunction following acute myocardial infarction in diabetic rats. Journal of cardiovascular translational research, 10(2), 209-220.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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