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Mouse Anti-AKT1S1 Recombinant Antibody (V2-6101) (CBMAB-0046CQ)

This product is a rabbit antibody that recognizes AKT1S1. The antibody BA0314 can be used for immunoassay techniques such as: IP, WB.
See all AKT1S1 antibodies
Published Data
Mouse Anti-AKT1S1 Recombinant Antibody (BA0314) in WB
Phosphorylation patterns for PRAS40 and WNK1 were successfully validated using immunoblotting (N = 3). ...View More
Pio, G. M., Xia, Y., Piaseczny, M. M., Chu, J. E., & Allan, A. L. (2017). Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells. PLoS One, 12(5), e0177640.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-6101
Antibody Isotype
IgG1
Application
WB, IF/ICC

Basic Information

Immunogen
E. coli-dervied recombinant human PRAS40 Gln119-Tyr256.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 μg/ml
ICC5-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AKT1 Substrate 1
Introduction
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein when phosphorylated. Akt1s1, which they called Pras40, from rat hepatoma cells. By EST database analysis, they identified mouse and human PRAS40. The deduced 256-amino acid human protein contains 2 N-terminal proline-rich regions and a C-terminal AKT phosphorylation site, thr246, that is conserved in rat and mouse. mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Entrez Gene ID
Human84335
Mouse67605
Rat292887
UniProt ID
HumanQ96B36
MouseQ9D1F4
RatD3ZH75
Alternative Names
AKT1 Substrate 1 (Proline-Rich); 40 KDa Proline-Rich AKT Substrate; PRAS40; Proline-Rich Akt Substrate; 40 KDa; Lobe
Function
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Biological Process
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: InterPro
Regulation of apoptotic process Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of neuron apoptotic process Source: UniProtKB
Cellular Location
Cytosol. Found in the cytosolic fraction of the brain.
Involvement in disease
Phosphorylated by AKT1 (PubMed:12524439). Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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