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Rabbit Anti-AKT1S1 (phospho T246) Recombinant Antibody (V2-6104) (CBMAB-0049CQ)

This product is a rabbit antibody that recognizes AKT1S1 (phospho T246). The antibody BA0306 can be used for immunoassay techniques such as: WB, IP.
See all AKT1S1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-6104
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Phosphopeptide containing the human PRAS40 T246 site.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Introduction
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein when phosphorylated. Akt1s1, which they called Pras40, from rat hepatoma cells. By EST database analysis, they identified mouse and human PRAS40. The deduced 256-amino acid human protein contains 2 N-terminal proline-rich regions and a C-terminal AKT phosphorylation site, thr246, that is conserved in rat and mouse. mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Entrez Gene ID
Human84335
Mouse67605
UniProt ID
HumanQ96B36
MouseQ9D1F4
Alternative Names
AKT1 Substrate 1 (Proline-Rich); 40 KDa Proline-Rich AKT Substrate; PRAS40; Proline-Rich Akt Substrate; 40 KDa; Lobe
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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