Mouse Anti-ALPP Recombinant Antibody (11F7) (CBMAB-A2438-YC)

Name: Mouse Anti-ALPP Recombinant Antibody (11F7)
SKU: CBMAB-A2438-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

11F7

Application

IF, IP, WB

Immunogen

Hep-2 cells expressing PLAP surface protein of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alkaline Phosphatase, Placental

Introduction

ALPP is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site c

Entrez Gene ID

250

UniProt ID

P05187

Alternative Names

PALP; PLAP; ALP; PLAP-1

Biological Process

Dephosphorylation Source: GO_Central

Cellular Location

Cell membrane

Topology

Helical: 513-529 aa

Chen, J., Chen, Z., Liu, M., Qiu, T., Feng, D., Zhao, C., ... & Xu, J. (2020). Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP. Mbio, 11(5), e01716-20.

Vimalraj, S. (2020). Alkaline phosphatase: structure, expression and its function in bone mineralization. Gene, 754, 144855.

Fushimi, A., Takeyama, H., Tachibana, T., & Manome, Y. (2020). Osteogenic cocktail induces calcifications in human breast cancer cell line via placental alkaline phosphatase expression. Scientific reports, 10(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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