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Mouse Anti-ALPP Recombinant Antibody (8B6) (CBMAB-A2446-YC)

Provided herein is a Mouse monoclonal antibody against Human Alkaline Phosphatase, Placental. The antibody can be used for immunoassay techniques, such as WB, FC, ELISA, IF, IHC-P, IHC-Fr.
See all ALPP antibodies
Published Data
Fig.1 A representative blot of Niemann-Pick C1-Like 1 (NPC1L1) protein expression in Caco2 cells
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Fig.2 Images of PLAP staining (green) on cell plasma membrane.
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Fig.3 Removal of PLAP and fluorescent antibody binding by acid washing.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
8B6
Antibody Isotype
IgG2a, κ
Application
WB, IHC, IF, IP

Basic Information

Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alkaline Phosphatase, Placental
Introduction
ALPP is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site c
Entrez Gene ID
250
UniProt ID
P05187
Alternative Names
Alkaline Phosphatase, Placental; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; PLAP; Alkaline Phosphatase, Placental (Regan Isozyme);
Biological Process
Dephosphorylation Source: GO_Central
Cellular Location
Cell membrane
Topology
Helical: 513-529 aa
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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