Rabbit Anti-ALPP Recombinant Antibody (
5B9) (V2LY-0725-LY1017)

Name: Rabbit Anti-ALPP Recombinant Antibody (5B9)
SKU: V2LY-0725-LY1017
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ALPP Recombinant Antibody (5B9) (V2LY-0725-LY1017) in WB

Western Blot
Positive WB detected in: PC-3 whole cell lysate,Hela whole cell lysate
All lanes: Placental alkaline phosphatase (PLAP) antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa

Rabbit Anti-ALPP Recombinant Antibody (5B9) (V2LY-0725-LY1017) in IHC

IHC image of V2LY-0725-LY1017 diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.39% DAB.

Rabbit Anti-ALPP Recombinant Antibody (5B9) (V2LY-0725-LY1017) in FC

Overlay Peak curve showing HepG2 cells surface stained with V2LY-0725-LY1017 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

5B9

Application

ELISA, WB, IHC, FC

Immunogen

A synthesized peptide derived from Human ALPP.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Alkaline Phosphatase, Placental

Entrez Gene ID

250

UniProt ID

P05187

Biological Process

Dephosphorylation Source: GO_Central

Cellular Location

Cell membrane

Topology

Helical: 513-529 aa

Chen, J., Chen, Z., Liu, M., Qiu, T., Feng, D., Zhao, C., ... & Xu, J. (2020). Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP. Mbio, 11(5), e01716-20.

Vimalraj, S. (2020). Alkaline phosphatase: structure, expression and its function in bone mineralization. Gene, 754, 144855.

Fushimi, A., Takeyama, H., Tachibana, T., & Manome, Y. (2020). Osteogenic cocktail induces calcifications in human breast cancer cell line via placental alkaline phosphatase expression. Scientific reports, 10(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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