Mouse Anti-ANXA4 Recombinant Antibody (H2) (CBMAB-A2920-YC)

Name: Mouse Anti-ANXA4 Recombinant Antibody (H2)
SKU: CBMAB-A2920-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, WB, IF, IP

Immunogen

Amino acids 107-139 within an internal region of Annexin IV of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Annexin A4

Introduction

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocyto

Entrez Gene ID

307

UniProt ID

P09525

Alternative Names

Annexin A4; Carbohydrate-Binding Protein P33/P41; Placental Anticoagulant Protein II; 35-Beta Calcimedin; Chromobindin-4; Lipocortin IV; Endonexin I; Protein II; Annexin-4; PAP-II; P32.5;

Function

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

Biological Process

Epithelial cell differentiation Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of interleukin-8 production Source: BHF-UCL
Negative regulation of NF-kappaB transcription factor activity Source: BHF-UCL
Notch signaling pathway Source: Ensembl
Regulation of transcription by RNA polymerase II Source: BHF-UCL
Signal transduction Source: UniProtKB

Cellular Location

Extracellular exosome; Nuclear membrane; Nucleus; Plasma membrane; Cell surface; Collagen-containing extracellular matrix; Cytoplasm; Perinuclear region of cytoplasm; Vesicle membrane

Li, N., Xi, Y., Du, H., Zhou, H., & Xu, Z. (2021). Annexin A4 is dispensable for hair cell development and function. Frontiers in cell and developmental biology, 9.

Florentsen, C. D., Kamp-Sonne, A., Moreno-Pescador, G., Pezeshkian, W., Zanjani, A. A. H., Khandelia, H., ... & Bendix, P. M. (2021). Annexin A4 trimers are recruited by high membrane curvatures in Giant Plasma Membrane Vesicles. Soft Matter, 17(2), 308-318.

Peng, Y., Zhang, Z., Zhang, A., Liu, C., Sun, Y., Peng, Z., & Liu, Y. (2021). Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma. Aging (Albany NY), 13(7), 10312.

Mularski, A., Sønder, S. L., Heitmann, A. S. B., Nylandsted, J., & Simonsen, A. C. (2021). Simultaneous membrane binding of Annexin A4 and A5 suppresses 2D lattice formation while maintaining curvature induction. Journal of Colloid and Interface Science, 600, 854-864.

Saad, Z. M., Fouad, Y., Ali, L. H., & Hassanin, T. M. (2020). Clinical Significance of Annexin A4 as a Biomarker in the Early Diagnosis of Hepatocellular Carcinoma. Asian Pacific Journal of Cancer Prevention: APJCP, 21(9), 2661.

Heinick, A., Pluteanu, F., Hermes, C., Klemme, A., Domnik, M., Husser, X., ... & Müller, F. U. (2020). Annexin A4 N‐terminal peptide inhibits adenylyl cyclase 5 and limits β‐adrenoceptor‐mediated prolongation of cardiac action potential. The FASEB Journal, 34(8), 10489-10504.

Nakayama, M., Miyagawa, H., Kuranami, Y., Tsunooka‐Ota, M., Yamaguchi, Y., & Kojima‐Aikawa, K. (2020). Annexin A4 inhibits sulfatide‐induced activation of coagulation factor XII. Journal of Thrombosis and Haemostasis, 18(6), 1357-1369.

Xu, Y., Sui, L., Qiu, B., Yin, X., Liu, J., & Zhang, X. (2019). ANXA4 promotes trophoblast invasion via the PI3K/Akt/eNOS pathway in preeclampsia. American Journal of Physiology-Cell Physiology, 316(4), C481-C491.

Zheng, M. D., Wang, N. D., Li, X. L., Yan, J., Tang, J. H., Zhao, X. H., & Zhang, Z. (2018). Toosendanin mediates cisplatin sensitization through targeting Annexin A4/ATP7A in non-small cell lung cancer cells. Journal of natural medicines, 72(3), 724-733.

Boye, T. L., Maeda, K., Pezeshkian, W., Sønder, S. L., Haeger, S. C., Gerke, V., ... & Nylandsted, J. (2017). Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair. Nature communications, 8(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ANXA4 Matched Antibody Pair (081) (CAT#: APMAB-081LY)

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Mouse Anti-ANXA4 Recombinant Antibody (A2921) (CAT#: CBMAB-A2921-YC)

ANXA4 Matched Antibody Pair (081) (CAT#: APMAB-081LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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