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Mouse Anti-ANXA5 Recombinant Antibody (1F4-1A5) (CBMAB-A2926-YC)

Provided herein is a Mouse monoclonal antibody against Human Annexin A5. The antibody can be used for immunoassay techniques, such as ELISA, IF, IHC-P, IP, WB.
See all ANXA5 antibodies
Published Data
Fig.1 Immunoblot of annexin V.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1F4-1A5
Antibody Isotype
IgG1
Application
ELISA, IF, IHC-P, IP, WB

Basic Information

Immunogen
ANXA5 (AAH01429, 1-321 aa) full length recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml
IHC-P3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-321

Target

Full Name
Annexin A5
Introduction
ANXA5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory
Entrez Gene ID
308
UniProt ID
P08758
Alternative Names
Annexin A5; Placental Anticoagulant Protein 4; Placental Anticoagulant Protein I; Vascular Anticoagulant-Alpha; Thromboplastin Inhibitor; Calphobindin I; Anchorin CII; Endonexin II; Lipocortin V; Annexin V; Annexin-5;
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Biological Process
Blood coagulation Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of coagulation Source: InterPro
Platelet degranulation Source: Reactome
Signal transduction Source: UniProtKB
Cellular Location
Cytosol; Extracellular exosome; Extracellular region; Collagen-containing extracellular matrix; Cytoplasm; Focal adhesion; Membrane
Involvement in disease
Pregnancy loss, recurrent, 3 (RPRGL3): A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.
PTM
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Haeckel, A., Ascher, L., Beindorff, N., Prasad, S., Garczyńska, K., Guo, J., & Schellenberger, E. (2021). Long-circulating XTEN864-annexin A5 fusion protein for phosphatidylserine-related therapeutic applications. Apoptosis, 1-14.

Dryllis, G., Giannopoulos, A., Zoi, C., Pouliakis, A., Logothetis, E., Voulgarelis, M., ... & Politou, M. (2020). Correlation of single nucleotide polymorphisms in the promoter region of the ANXA5 (annexin A5) gene with recurrent miscarriages in women of Greek origin. The Journal of Maternal-Fetal & Neonatal Medicine, 33(9), 1538-1543.

Bolean, M., Izzi, B., Bottini, M., Ramos, A. P., Millán, J. L., Hoylaerts, M. F., & Ciancaglini, P. (2020). Matrix vesicle biomimetics harboring Annexin A5 and alkaline phosphatase bind to the native collagen matrix produced by mineralizing vascular smooth muscle cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1864(8), 129629.

Terashima, R., Saigo, T., Laoharatchatathanin, T., Kurusu, S., Brachvogel, B., Pöschl, E., & Kawaminami, M. (2020). Augmentation of Nr4a3 and suppression of Fshb expression in the pituitary gland of female Annexin A5 null mouse. Journal of the Endocrine Society, 4(9), bvaa096.

Kang, T. H., Park, J. H., Yang, A., Park, H. J., Lee, S. E., Kim, Y. S., ... & Hung, C. F. (2020). Annexin A5 as an immune checkpoint inhibitor and tumor-homing molecule for cancer treatment. Nature communications, 11(1), 1-16.

Yu, C., Muñoz, L. E., Mallavarapu, M., Herrmann, M., & Finnemann, S. C. (2019). Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis. Journal of cell science, 132(20), jcs232439.

Danisik, H., Bogdanova, N., & Markoff, A. (2019). Micromolar zinc in Annexin A5 anticoagulation as a potential remedy for RPRGL3-associated recurrent pregnancy loss. Reproductive Sciences, 26(3), 348-356.

de Jong, R. C., Pluijmert, N. J., de Vries, M. R., Pettersson, K., Atsma, D. E., Jukema, J. W., & Quax, P. H. (2018). Annexin A5 reduces infarct size and improves cardiac function after myocardial ischemia-reperfusion injury by suppression of the cardiac inflammatory response. Scientific reports, 8(1), 1-10.

Zhang, B., Zeng, X., Xu, Y., Zhang, Y., Huang, N., Gu, Y., ... & Liu, X. (2017). The association between annexin A5 (ANXA5) gene polymorphism and left ventricular hypertrophy (LVH) in Chinese endogenous hypertension patients. Medicine, 96(44).

Baek, H. S., Park, N., Kwon, Y. J., Ye, D. J., Shin, S., & Chun, Y. J. (2017). Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells. Oncotarget, 8(43), 74263.

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For research use only. Not intended for any clinical use.

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