Mouse Anti-ANXA5 Recombinant Antibody (CBYC-A636) (CBMAB-A2934-YC)

Name: Mouse Anti-ANXA5 Recombinant Antibody (CBYC-A636)
SKU: CBMAB-A2934-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ANXA5 Recombinant Antibody (CBYC-A636) in WB

Western blot analysis of heat shock protein beta-1 (HSPB1) and annexin A2 (ANXA2) in trachea tissue, annexin A5 (ANXA5) in kidney tissue of chickens infected with the IBV ck/CH/LDL/97I P5 and ck/CH/LDL/97I P115 strain. ...View More

Cao, Z., Han, Z., Shao, Y., Liu, X., Sun, J., Yu, D., ... & Liu, S. (2012). Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo. Proteome Science, 10, 1-19.

Mouse Anti-ANXA5 Recombinant Antibody (CBYC-A636) in WB

Western blots of tumor related proteins. The six tumor related proteins are Bax, Keratin 19, Histone H1 (H1.1 and H1.2), E-Cadherin, Beta Tubulin. Note: this figure includes two proteins common to tumor and stroma, namely, Vimentin and Annexin A5. MW marker (1), blot (2). ...View More

Johann Jr, D. J., Rodriguez-Canales, J., Mukherjee, S., Prieto, D. A., Hanson, J. C., Emmert-Buck, M., & Blonder, J. (2009). Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry. Journal of proteome research, 8(5), 2310-2318.

Mouse Anti-ANXA5 Recombinant Antibody (CBYC-A636) in WB

Western blot analysis for a subset of differentially expressed proteins
The left panel shows Western blot results with the right panel showing MS/MS spectra of a peptide from the corresponding protein. ...View More

Molina, H., Yang, Y., Ruch, T., Kim, J. W., Mortensen, P., Otto, T., ... & Pandey, A. (2009). Temporal profiling of the adipocyte proteome during differentiation using a five-plex SILAC based strategy. Journal of proteome research, 8(1), 48-58.

Mouse Anti-ANXA5 Recombinant Antibody (CBYC-A636) in IHC

Histological sections of myocardial biopsy specimens from one normotensive subject (A), one hypertensive patient with no identified cardiac structural and functional abnormalities (B), one patient with asymptomatic left ventricular hypertrophy (C), and one heart failure hypertensive patient with ejection fraction ,0.40 (D-H). Immunostaining for Annexin A5 is shown in brown (arrows). (I and J ) Negative controls for primary antibody omission and primary antibody preadsorption, respectively. (Magnification 100). ...View More

Ravassa, S., González, A., López, B., Beaumont, J., Querejeta, R., Larman, M., & Díez, J. (2007). Upregulation of myocardial Annexin A5 in hypertensive heart disease: association with systolic dysfunction. European heart journal, 28(22), 2785-2791.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A636

Application

WB, IP

Immunogen

Recombinant avian Annexin V.

Host Species

Mouse

Specificity

Human, Bird

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Annexin A5

Introduction

ANXA5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory

Entrez Gene ID

308

UniProt ID

P08758

Alternative Names

ANX5; ENX2; HEL-S-7; PP4; RPRGL3

Function

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Biological Process

Blood coagulation Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of coagulation Source: InterPro
Platelet degranulation Source: Reactome
Signal transduction Source: UniProtKB

Cellular Location

Cytosol; Extracellular exosome; Extracellular region; Collagen-containing extracellular matrix; Cytoplasm; Focal adhesion; Membrane

Involvement in disease

Pregnancy loss, recurrent, 3 (RPRGL3): A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.

PTM

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Haeckel, A., Ascher, L., Beindorff, N., Prasad, S., Garczyńska, K., Guo, J., & Schellenberger, E. (2021). Long-circulating XTEN864-annexin A5 fusion protein for phosphatidylserine-related therapeutic applications. Apoptosis, 1-14.

Dryllis, G., Giannopoulos, A., Zoi, C., Pouliakis, A., Logothetis, E., Voulgarelis, M., ... & Politou, M. (2020). Correlation of single nucleotide polymorphisms in the promoter region of the ANXA5 (annexin A5) gene with recurrent miscarriages in women of Greek origin. The Journal of Maternal-Fetal & Neonatal Medicine, 33(9), 1538-1543.

Bolean, M., Izzi, B., Bottini, M., Ramos, A. P., Millán, J. L., Hoylaerts, M. F., & Ciancaglini, P. (2020). Matrix vesicle biomimetics harboring Annexin A5 and alkaline phosphatase bind to the native collagen matrix produced by mineralizing vascular smooth muscle cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1864(8), 129629.

Terashima, R., Saigo, T., Laoharatchatathanin, T., Kurusu, S., Brachvogel, B., Pöschl, E., & Kawaminami, M. (2020). Augmentation of Nr4a3 and suppression of Fshb expression in the pituitary gland of female Annexin A5 null mouse. Journal of the Endocrine Society, 4(9), bvaa096.

Kang, T. H., Park, J. H., Yang, A., Park, H. J., Lee, S. E., Kim, Y. S., ... & Hung, C. F. (2020). Annexin A5 as an immune checkpoint inhibitor and tumor-homing molecule for cancer treatment. Nature communications, 11(1), 1-16.

Yu, C., Muñoz, L. E., Mallavarapu, M., Herrmann, M., & Finnemann, S. C. (2019). Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis. Journal of cell science, 132(20), jcs232439.

Danisik, H., Bogdanova, N., & Markoff, A. (2019). Micromolar zinc in Annexin A5 anticoagulation as a potential remedy for RPRGL3-associated recurrent pregnancy loss. Reproductive Sciences, 26(3), 348-356.

de Jong, R. C., Pluijmert, N. J., de Vries, M. R., Pettersson, K., Atsma, D. E., Jukema, J. W., & Quax, P. H. (2018). Annexin A5 reduces infarct size and improves cardiac function after myocardial ischemia-reperfusion injury by suppression of the cardiac inflammatory response. Scientific reports, 8(1), 1-10.

Zhang, B., Zeng, X., Xu, Y., Zhang, Y., Huang, N., Gu, Y., ... & Liu, X. (2017). The association between annexin A5 (ANXA5) gene polymorphism and left ventricular hypertrophy (LVH) in Chinese endogenous hypertension patients. Medicine, 96(44).

Baek, H. S., Park, N., Kwon, Y. J., Ye, D. J., Shin, S., & Chun, Y. J. (2017). Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells. Oncotarget, 8(43), 74263.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ANXA5 Matched Antibody Pair (082) (CAT#: APMAB-082LY)

ANXA5 Matched Antibody Pair (083) (CAT#: APMAB-083LY)

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For research use only. Not intended for any clinical use.

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