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Mouse Anti-ANXA5 Recombinant Antibody (CF030) (CBMAB-FT458LY)

The product is antibody recognizes ANXA5. The antibody CF030 immunoassay techniques such as: ELISA, WB, IHC, IF.
See all ANXA5 antibodies
Published Data
Mouse Anti-ANXA5 Recombinant Antibody (CF030) in WB
Western blotting showed the positive expression of the exosome marker proteins Annexin V, CD9, Tsg101, and CD63 in the exosome group. ...View More
Tong, L., Yan, C., Wang, M., Yang, J., Wang, H., & Wang, Y. (2021). Prognostic value of serum exosomal AHCY expression in hepatitis B-induced liver cirrhosis. Frontiers in Medicine, 8, 777452.
Mouse Anti-ANXA5 Recombinant Antibody (CF030) in WB
Induction of thrombospondin-1 and tenascin C by aesculetin temporal induction of annexin V and PHOSPHO 1, and elevation of annexin V and PHOSPHO 1 with aesculetin. ...View More
Na, W., Kang, M. K., Park, S. H., Kim, D. Y., Oh, S. Y., Oh, M. S., ... & Kang, Y. H. (2021). Aesculetin accelerates osteoblast differentiation and matrix-vesicle-mediated mineralization. International Journal of Molecular Sciences, 22(22), 12391.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CF030
Antibody Isotype
IgG2a, κ
Application
WB, IP, ICC, ELISA

Basic Information

Immunogen
Amino acids 298-319 C-terminus (h) of Annexin V of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Annexin A5
Introduction
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human308
Mouse11747
UniProt ID
HumanP08758
MouseP48036
Alternative Names
Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Biological Process
Blood coagulation Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of coagulation Source: InterPro
Platelet degranulation Source: Reactome
Signal transduction Source: UniProtKB
Cellular Location
Cytosol; Extracellular exosome; Extracellular region; Collagen-containing extracellular matrix; Cytoplasm; Focal adhesion; Membrane
Involvement in disease
Pregnancy loss, recurrent, 3 (RPRGL3): A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.
PTM
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Haeckel, A., Ascher, L., Beindorff, N., Prasad, S., Garczyńska, K., Guo, J., & Schellenberger, E. (2021). Long-circulating XTEN864-annexin A5 fusion protein for phosphatidylserine-related therapeutic applications. Apoptosis, 1-14.

Dryllis, G., Giannopoulos, A., Zoi, C., Pouliakis, A., Logothetis, E., Voulgarelis, M., ... & Politou, M. (2020). Correlation of single nucleotide polymorphisms in the promoter region of the ANXA5 (annexin A5) gene with recurrent miscarriages in women of Greek origin. The Journal of Maternal-Fetal & Neonatal Medicine, 33(9), 1538-1543.

Bolean, M., Izzi, B., Bottini, M., Ramos, A. P., Millán, J. L., Hoylaerts, M. F., & Ciancaglini, P. (2020). Matrix vesicle biomimetics harboring Annexin A5 and alkaline phosphatase bind to the native collagen matrix produced by mineralizing vascular smooth muscle cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1864(8), 129629.

Terashima, R., Saigo, T., Laoharatchatathanin, T., Kurusu, S., Brachvogel, B., Pöschl, E., & Kawaminami, M. (2020). Augmentation of Nr4a3 and suppression of Fshb expression in the pituitary gland of female Annexin A5 null mouse. Journal of the Endocrine Society, 4(9), bvaa096.

Kang, T. H., Park, J. H., Yang, A., Park, H. J., Lee, S. E., Kim, Y. S., ... & Hung, C. F. (2020). Annexin A5 as an immune checkpoint inhibitor and tumor-homing molecule for cancer treatment. Nature communications, 11(1), 1-16.

Yu, C., Muñoz, L. E., Mallavarapu, M., Herrmann, M., & Finnemann, S. C. (2019). Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis. Journal of cell science, 132(20), jcs232439.

Danisik, H., Bogdanova, N., & Markoff, A. (2019). Micromolar zinc in Annexin A5 anticoagulation as a potential remedy for RPRGL3-associated recurrent pregnancy loss. Reproductive Sciences, 26(3), 348-356.

de Jong, R. C., Pluijmert, N. J., de Vries, M. R., Pettersson, K., Atsma, D. E., Jukema, J. W., & Quax, P. H. (2018). Annexin A5 reduces infarct size and improves cardiac function after myocardial ischemia-reperfusion injury by suppression of the cardiac inflammatory response. Scientific reports, 8(1), 1-10.

Zhang, B., Zeng, X., Xu, Y., Zhang, Y., Huang, N., Gu, Y., ... & Liu, X. (2017). The association between annexin A5 (ANXA5) gene polymorphism and left ventricular hypertrophy (LVH) in Chinese endogenous hypertension patients. Medicine, 96(44).

Baek, H. S., Park, N., Kwon, Y. J., Ye, D. J., Shin, S., & Chun, Y. J. (2017). Annexin A5 suppresses cyclooxygenase-2 expression by downregulating the protein kinase C-ζ–nuclear factor-κB signaling pathway in prostate cancer cells. Oncotarget, 8(43), 74263.

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For research use only. Not intended for any clinical use.

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