Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116) (CBMAB-1085-CN)

Name: Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116)
SKU: CBMAB-1085-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116) in Immunoblot

Effects of HDACi and HSP90i incubation or preincubation prior to cisplatin on protein expression levels of pro-/antiapoptotic proteins. Representative immunoblot analysis of APAF-1, p21, survivin and α-tubulin (as loading control) in A2780 and A2780CisR cells is shown. For combination treatments, panobinostat (pano) and/or luminespib (lumi) were administrated 48 h prior to 24 h cisplatin treatment with respective IC50 concentrations. Cell line specific control was treated with vehicle for 72 h. ...View More

Rodrigues Moita, A. J., Bandolik, J. J., Hansen, F. K., Kurz, T., Hamacher, A., & Kassack, M. U. (2020). Priming with HDAC inhibitors sensitizes ovarian cancer cells to treatment with cisplatin and HSP90 inhibitors. International Journal of Molecular Sciences, 21(21), 8300.

Mouse Anti-APAF1 Recombinant Antibody (CBLNA-116) in Immunoblot

832/13 and primary rat islets were untreated (no virus) or treated with recombinant adenoviruses containing cDNAs corresponding to human Apaf-1, human caspase 3, human caspase 9 or the control gene, β-galactosidase (β-gal), as indicated. 72 h post-infection, 832/13 cells (A) and primary rat islets (B) were treated with DMSO (control) or thapsigargin (100 nM or 1 μM, respectively) for 18 h. ...View More

Hayes, H. L., Peterson, B. S., Haldeman, J. M., Newgard, C. B., Hohmeier, H. E., & Stephens, S. B. (2017). Delayed apoptosis allows islet β-cells to implement an autophagic mechanism to promote cell survival. PloS one, 12(2), e0172567.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLNA-116

Application

Immunogen

E. coli-derived recombinant human APAF-1 Leu10-Asp214.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apoptotic Peptidase Activating Factor 1

Introduction

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Entrez Gene ID

317

UniProt ID

O14727

Alternative Names

CED4; APAF-1

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
Aging Source: Ensembl
Apoptotic process Source: ProtInc
Cardiac muscle cell apoptotic process Source: Ensembl
Cell differentiation Source: Ensembl
Cellular response to transforming growth factor beta stimulus Source: Ensembl
Forebrain development Source: Ensembl
Intrinsic apoptotic signaling pathway Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: Ensembl
Kidney development Source: Ensembl
Negative regulation of G0 to G1 transition Source: Reactome
Nervous system development Source: ProtInc
Neural tube closure Source: Ensembl
Neuron apoptotic process Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic signaling pathway Source: Ensembl
Regulation of apoptotic DNA fragmentation Source: Ensembl
Regulation of apoptotic process Source: UniProtKB
Response to G1 DNA damage checkpoint signaling Source: UniProtKB
Response to hypoxia Source: Ensembl
Response to nutrient Source: Ensembl

Cellular Location

Cytoplasm

Cao, Y., Gao, W., Tang, H., Wang, T., & You, C. (2021). Long Non-coding RNA TALNEC2 Aggravates Cerebral Ischemia/Reperfusion Injury via Acting as a competing endogenous RNAs for miR-650 to target apoptotic peptidase activating factor 1. Neuroscience, 458, 64-76.

Lukosiute-Urboniene, A., Mazeike, A., Kazokaite, M., Silkuniene, G., Silkunas, M., Barauskas, V., ... & Dambrauskas, Z. (2020). Epigenetic regulation of APAF-1 through DNA methylation in pancreatic cancer. Anticancer Research, 40(7), 3765-3779.

Cao, Y., Gao, W., Tang, H., Wang, T., & You, C. (2020). LncRNA TALNEC2 aggravates cerebral ischemia/reperfusion injury via acting as a ceRNA for miR-650 to target apoptotic peptidase activating factor 1. Neuroscience.

Li, M. L., Lin, J. Y., Chen, B. S., Weng, K. F., Shih, S. R., Calderon, J. D., ... & Brewer, G. (2019). EV71 3C protease induces apoptosis by cleavage of hnRNP A1 to promote apaf-1 translation. PloS one, 14(9), e0221048.

Bugueno, I. M., Batool, F., Korah, L., Benkirane-Jessel, N., & Huck, O. (2018). Porphyromonas gingivalis differentially modulates apoptosome apoptotic peptidase activating factor 1 in epithelial cells and fibroblasts. The American journal of pathology, 188(2), 404-416.

Ratn, A., Awasthi, Y., Kumar, M., Singh, S. K., Tripathi, R., & Trivedi, S. P. (2017). Phorate induced oxidative stress, DNA damage and differential expression of p53, apaf-1 and cat genes in fish, Channa punctatus (Bloch, 1793). Chemosphere, 182, 382-391.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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