Mouse Anti-APAF1 Recombinant Antibody (CBT3095) (V2LY-0625-LY904)

Name: Mouse Anti-APAF1 Recombinant Antibody (CBT3095)
SKU: V2LY-0625-LY904
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT3095

Antibody Isotype

IgG2b

Application

Basic Information

Immunogen

Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Apoptotic Peptidase Activating Factor 1

Entrez Gene ID

317

UniProt ID

O14727

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
Aging Source: Ensembl
Apoptotic process Source: ProtInc
Cardiac muscle cell apoptotic process Source: Ensembl
Cell differentiation Source: Ensembl
Cellular response to transforming growth factor beta stimulus Source: Ensembl
Forebrain development Source: Ensembl
Intrinsic apoptotic signaling pathway Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: Ensembl
Kidney development Source: Ensembl
Negative regulation of G0 to G1 transition Source: Reactome
Nervous system development Source: ProtInc
Neural tube closure Source: Ensembl
Neuron apoptotic process Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic signaling pathway Source: Ensembl
Regulation of apoptotic DNA fragmentation Source: Ensembl
Regulation of apoptotic process Source: UniProtKB
Response to G1 DNA damage checkpoint signaling Source: UniProtKB
Response to hypoxia Source: Ensembl
Response to nutrient Source: Ensembl

Cellular Location

Cytoplasm

More Infomation

References

Cao, Y., Gao, W., Tang, H., Wang, T., & You, C. (2021). Long Non-coding RNA TALNEC2 Aggravates Cerebral Ischemia/Reperfusion Injury via Acting as a competing endogenous RNAs for miR-650 to target apoptotic peptidase activating factor 1. Neuroscience, 458, 64-76.

Lukosiute-Urboniene, A., Mazeike, A., Kazokaite, M., Silkuniene, G., Silkunas, M., Barauskas, V., ... & Dambrauskas, Z. (2020). Epigenetic regulation of APAF-1 through DNA methylation in pancreatic cancer. Anticancer Research, 40(7), 3765-3779.

Cao, Y., Gao, W., Tang, H., Wang, T., & You, C. (2020). LncRNA TALNEC2 aggravates cerebral ischemia/reperfusion injury via acting as a ceRNA for miR-650 to target apoptotic peptidase activating factor 1. Neuroscience.

Li, M. L., Lin, J. Y., Chen, B. S., Weng, K. F., Shih, S. R., Calderon, J. D., ... & Brewer, G. (2019). EV71 3C protease induces apoptosis by cleavage of hnRNP A1 to promote apaf-1 translation. PloS one, 14(9), e0221048.

Bugueno, I. M., Batool, F., Korah, L., Benkirane-Jessel, N., & Huck, O. (2018). Porphyromonas gingivalis differentially modulates apoptosome apoptotic peptidase activating factor 1 in epithelial cells and fibroblasts. The American journal of pathology, 188(2), 404-416.

Ratn, A., Awasthi, Y., Kumar, M., Singh, S. K., Tripathi, R., & Trivedi, S. P. (2017). Phorate induced oxidative stress, DNA damage and differential expression of p53, apaf-1 and cat genes in fish, Channa punctatus (Bloch, 1793). Chemosphere, 182, 382-391.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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