Mouse Anti-ARHGDIA Recombinant Antibody (13B47) (CBMAB-A3507-YC)

Name: Mouse Anti-ARHGDIA Recombinant Antibody (13B47)
SKU: CBMAB-A3507-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARHGDIA Recombinant Antibody (13B47) CBMAB-A3507-YC in IP

Elevated RhoC NDU disrupts its interaction of total RhoC with RhoGDIα in vivo. RhoGDIα immunoprecipitation from Lis1fl/+ or Lis1fl/− brain shows decreased RhoC binding, while RhoA and Rac3 remain unchanged. Graph quantifies results from n = 3 biologically independent experiments done in duplicate, *P < 0.0312, ns = not significant with P = 0.4375 (RhoA) and P < 0.9999 (Rac3). Data are shown as the mean ± s.d. ...View More

Kholmanskikh, S., Singh, S., & Ross, M. E. (2022). Activation of RhoC by regulatory ubiquitination is mediated by LNX1 and suppressed by LIS1. Scientific Reports, 12(1), 16493.

Mouse Anti-ARHGDIA Recombinant Antibody (13B47) CBMAB-A3507-YC in IF

Images of RhoGDI-GFP and Y156 phosphomutants (Y>E and Y>F) expressed in WT cells, fixed and stained for E-cadherin. ...View More

Howden, J. D., Michael, M., Hight-Warburton, W., & Parsons, M. (2021). α2β1 integrins spatially restrict Cdc42 activity to stabilise adherens junctions. BMC biology, 19(1), 1-14.

Mouse Anti-ARHGDIA Recombinant Antibody (13B47) CBMAB-A3507-YC in IF

Co-localization of Rac1-GDI was determined by immunofluorescence using Alexa-Flour 488 (green) conjugated and Texas Red (red) conjugated secondary antibodies for Rac1 and GDI respectively. ...View More

Mohammad, G., Duraisamy, A. J., Kowluru, A., & Kowluru, R. A. (2019). Functional regulation of an oxidative stress mediator, Rac1, in diabetic retinopathy. Molecular neurobiology, 56, 8643-8655.

Mouse Anti-ARHGDIA Recombinant Antibody (13B47) CBMAB-A3507-YC in WB

Protein expression of GDI was determined by western blot technique; the blot is a representative of 5-6 samples in each group. ...View More

Mohammad, G., Duraisamy, A. J., Kowluru, A., & Kowluru, R. A. (2019). Functional regulation of an oxidative stress mediator, Rac1, in diabetic retinopathy. Molecular neurobiology, 56, 8643-8655.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

13B47

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 2-29 at the N-terminus of Rho GDIα of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Rho GDP Dissociation Inhibitor Alpha

Introduction

ARHGDIA is a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Ac

Entrez Gene ID

Human	396
Mouse	192662
Monkey	714752

UniProt ID

Human	P52565
Mouse	Q99PT1
Monkey	F1PL93

Alternative Names

Rho GDP Dissociation Inhibitor Alpha; Rho GDP Dissociation Inhibitor (GDI) Alpha; GDIA1; GDP-Dissociation Inhibitor, Aplysia RAS-Related 1; Epididymis Secretory Sperm Binding Protein Li 47e; Rho GDP-Dissociation Inhibitor 1; Rho-GDI Alpha;

Function

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.

Biological Process

Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of axonogenesis Source: Reactome
Negative regulation of cell adhesion Source: ProtInc
Positive regulation of axonogenesis Source: Reactome
Positive regulation of GTPase activity Source: GOC
Regulation of actin cytoskeleton reorganization Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central
Semaphorin-plexin signaling pathway Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Nephrotic syndrome 8 (NPHS8): A form of nephrotic syndrome, a renal disease clinically characterized by progressive renal failure, severe proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show diffuse mesangial sclerosis, with small glomeruli, hypercellularity, increased extracellular matrix, and contracted/collapsed glomerular tufts surrounded by immature or abnormal podocytes.

Wei, Z., Xu, H., Zhang, Y., Yi, X., Yang, X., Chen, Y., ... & Yang, F. (2019). Rho GDP dissociation inhibitor α silencing attenuates silicosis by inhibiting RhoA/Rho kinase signalling. Experimental cell research, 380(2), 131-140.

Xie, F., Shao, S., Zhang, B., Wang, H., & Liu, B. (2017). Role of Rho-specific guanine nucleotide dissociation inhibitor α regulation in cell migration. Acta histochemica, 119(3), 183-189.

Sun, Y., Guo, C., Ma, P., Lai, Y., Yang, F., Cai, J., ... & Wu, C. (2017). Kindlin-2 association with Rho GDP-dissociation inhibitor α suppresses Rac1 activation and podocyte injury. Journal of the American Society of Nephrology, 28(12), 3545-3562.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ARHGDIA Matched Antibody Pair (098) (CAT#: APMAB-098LY)

ARHGDIA Matched Antibody Pair (099) (CAT#: APMAB-099LY)

ARHGDIA Matched Antibody Pair (100) (CAT#: APMAB-100LY)

ARHGDIA Matched Antibody Pair (101) (CAT#: APMAB-101LY)

Related Products

ARHGDIA Matched Antibody Pair (100) (CAT#: APMAB-100LY)

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Rabbit Anti-ARHGDIA Recombinant Antibody (CBCNR-485) (CAT#: CBMAB-R0294-CN)

ARHGDIA Matched Antibody Pair (101) (CAT#: APMAB-101LY)

Mouse Anti-ARHGDIA Recombinant Antibody (CBCNA-009) (CAT#: CBMAB-R0415-CN)

ARHGDIA Matched Antibody Pair (098) (CAT#: APMAB-098LY)

ARHGDIA Matched Antibody Pair (099) (CAT#: APMAB-099LY)

Mouse Anti-ARHGDIA Recombinant Antibody (R0411) (CAT#: CBMAB-R0411-CN)

Mouse Anti-ARHGDIA Recombinant Antibody (1E12-2G11) (CAT#: CBMAB-A3509-YC)

Mouse Anti-ARHGDIA Recombinant Antibody (1G5-2F3) (CAT#: CBMAB-A0460-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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