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Mouse Anti-ARHGDIA Recombinant Antibody (1G5-2F3) (CBMAB-A0460-LY)

The product is antibody recognizes ARHGDIA. The antibody 1G5-2F3 immunoassay techniques such as: WB, ELISA.
See all ARHGDIA antibodies
Published Data
Mouse Anti-ARHGDIA Recombinant Antibody (1G5-2F3) CBMAB-A0460-LY in WB
Western blot analysis of Rho GDI expression change. actin and Rho GDIwere probed with corresponding antibodies as described in text. ...View More
Liu, J., Huang, H., Xing, X., Xi, R., Zhuang, Z., Yuan, J., ... & Zhao, J. (2007). Comparative proteomic analysis on human L-02 liver cells treated with varying concentrations of trichloroethylene. Toxicology and industrial health, 23(2), 91-101.

Summary

Host Animal
Mouse
Specificity
Human
Clone
1G5-2F3
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ARHGDIA (AAH16031, 1 a.a. ~ 204 a.a) full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Rho GDP Dissociation Inhibitor Alpha
Introduction
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM
Entrez Gene ID
396
UniProt ID
P52565
Alternative Names
GDIA1; MGC117248; RHOGDI; RHOGDI-1
Function
Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Biological Process
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of axonogenesis Source: Reactome
Negative regulation of cell adhesion Source: ProtInc
Positive regulation of axonogenesis Source: Reactome
Positive regulation of GTPase activity Source: GOC
Regulation of actin cytoskeleton reorganization Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central
Semaphorin-plexin signaling pathway Source: UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Nephrotic syndrome 8 (NPHS8): A form of nephrotic syndrome, a renal disease clinically characterized by progressive renal failure, severe proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show diffuse mesangial sclerosis, with small glomeruli, hypercellularity, increased extracellular matrix, and contracted/collapsed glomerular tufts surrounded by immature or abnormal podocytes.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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