Mouse Anti-ARHGDIA Recombinant Antibody (CBT4082) (V2LY-0625-LY2741)

Name: Mouse Anti-ARHGDIA Recombinant Antibody (CBT4082)
SKU: V2LY-0625-LY2741
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ARHGDIA Recombinant Antibody (CBT4082) (V2LY-0625-LY2741) in WB

Mouse Anti-ARHGDIA Recombinant Antibody (CBT4082) (V2LY-0625-LY2741) in IF

Mouse Anti-ARHGDIA Recombinant Antibody (CBT4082) (V2LY-0625-LY2741) in FC

Mouse Anti-ARHGDIA Recombinant Antibody (CBT4082) (V2LY-0625-LY2741) in ELISA

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Monkey

Clone

CBT4082

Antibody Isotype

IgG1

Application

WB, ICC, FC

Basic Information

Immunogen

Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

396

UniProt ID

P52565

Function

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.

Biological Process

Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of axonogenesis Source: Reactome
Negative regulation of cell adhesion Source: ProtInc
Positive regulation of axonogenesis Source: Reactome
Positive regulation of GTPase activity Source: GOC
Regulation of actin cytoskeleton reorganization Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central
Semaphorin-plexin signaling pathway Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Nephrotic syndrome 8 (NPHS8): A form of nephrotic syndrome, a renal disease clinically characterized by progressive renal failure, severe proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show diffuse mesangial sclerosis, with small glomeruli, hypercellularity, increased extracellular matrix, and contracted/collapsed glomerular tufts surrounded by immature or abnormal podocytes.

More Infomation

References

Wei, Z., Xu, H., Zhang, Y., Yi, X., Yang, X., Chen, Y., ... & Yang, F. (2019). Rho GDP dissociation inhibitor α silencing attenuates silicosis by inhibiting RhoA/Rho kinase signalling. Experimental cell research, 380(2), 131-140.

Xie, F., Shao, S., Zhang, B., Wang, H., & Liu, B. (2017). Role of Rho-specific guanine nucleotide dissociation inhibitor α regulation in cell migration. Acta histochemica, 119(3), 183-189.

Sun, Y., Guo, C., Ma, P., Lai, Y., Yang, F., Cai, J., ... & Wu, C. (2017). Kindlin-2 association with Rho GDP-dissociation inhibitor α suppresses Rac1 activation and podocyte injury. Journal of the American Society of Nephrology, 28(12), 3545-3562.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

ARHGDIA Matched Antibody Pair (098) (CAT#: APMAB-098LY)

ARHGDIA Matched Antibody Pair (099) (CAT#: APMAB-099LY)

ARHGDIA Matched Antibody Pair (100) (CAT#: APMAB-100LY)

ARHGDIA Matched Antibody Pair (101) (CAT#: APMAB-101LY)

Related Products

Mouse Anti-ARHGDIA Recombinant Antibody (1G5-2F3) (CAT#: CBMAB-A0460-LY)

Mouse Anti-ARHGDIA Recombinant Antibody (CBCNA-009) (CAT#: CBMAB-R0415-CN)

ARHGDIA Matched Antibody Pair (099) (CAT#: APMAB-099LY)

ARHGDIA Matched Antibody Pair (101) (CAT#: APMAB-101LY)

Mouse Anti-ARHGDIA Recombinant Antibody (2G3) (CAT#: CBMAB-R0413-CN)

Rabbit Anti-ARHGDIA Recombinant Antibody (EG230) (CAT#: CBMAB-EN257-LY)

Rabbit Anti-ARHGDIA Recombinant Antibody (CBCNR-485) (CAT#: CBMAB-R0294-CN)

ARHGDIA Matched Antibody Pair (098) (CAT#: APMAB-098LY)

Mouse Anti-ARHGDIA Recombinant Antibody (R0411) (CAT#: CBMAB-R0411-CN)

ARHGDIA Matched Antibody Pair (100) (CAT#: APMAB-100LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

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