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Mouse Anti-ARHGEF1 Recombinant Antibody (4C4) (CBMAB-A0465-LY)

The product is antibody recognizes ARHGEF1. The antibody 4C4 immunoassay techniques such as: WB, ELISA.
See all ARHGEF1 antibodies
Published Data
Mouse Anti-ARHGEF1 Recombinant Antibody (4C4) CBMAB-A0465-LY in WB and IF
Expression of lsc/p115 in the esophagus. (A) Western blot analysis reveals lsc/p115 in the esophagus, stomach, colon, and spleen, but not in the small intestine. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading control. (B) RNA in situ hybridization reveals mRNA expression in the epithelial (arrows) and muscle layers (arrowheads); (C) sense probe control. (D) Immunofluorescence shows lsc/p115 (green) surrounding the -tubulin class III-positive neurons (red). (E) Lsc/p115 expression (red) overlaps with GFAP staining (green). Scale bars, 100 m. ...View More
Zizer, E., Beilke, S., Bäuerle, T., Schilling, K., Möhnle, U., Adler, G., ... & Wagner, M. (2010). Loss of Lsc/p115 protein leads to neuronal hypoplasia in the esophagus and an achalasia-like phenotype in mice. Gastroenterology, 139(4), 1344-1354.
Mouse Anti-ARHGEF1 Recombinant Antibody (4C4) CBMAB-A0465-LY in IHC
H&E staining shows the reduced thickness of the muscle layer in (right) lsc/p115-deficient mice compared with the (leift) control. ...View More
Zizer, E., Beilke, S., Bäuerle, T., Schilling, K., Möhnle, U., Adler, G., ... & Wagner, M. (2010). Loss of Lsc/p115 protein leads to neuronal hypoplasia in the esophagus and an achalasia-like phenotype in mice. Gastroenterology, 139(4), 1344-1354.

Summary

Host Animal
Mouse
Specificity
Human
Clone
4C4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA, IF

Basic Information

Immunogen
ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Rho Guanine Nucleotide Exchange Factor 1
Introduction
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq]
Entrez Gene ID
9138
UniProt ID
Q92888
Alternative Names
GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5
Function
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits (PubMed:9641915, PubMed:9641916).
Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase (PubMed:9641915, PubMed:9641916, PubMed:8810315, PubMed:30521495).
Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain (PubMed:9641916).
This GEF activity is inhibited by binding to activated GNA12 (PubMed:9641916).
Mediates angiotensin-2-induced RhoA activation (PubMed:20098430).
Biological Process
G protein-coupled receptor signaling pathway Source: Reactome
Positive regulation of apoptotic process Source: Reactome
Positive regulation of GTPase activity Source: GOC
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc
Cellular Location
Cytoplasm; Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.
Involvement in disease
Immunodeficiency 62 (IMD62): An autosomal recessive, primary immunologic disorder characterized by recurrent severe respiratory tract infections and bronchiectasis, due to antibody deficiency. Affected individuals have an abnormal B cell immunophenotype, with low levels of circulating memory B cells.
PTM
Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.

Gheyouche, E., Bagueneau, M., Loirand, G., Offmann, B., & Téletchéa, S. (2021). Structural Design and Analysis of the RHOA-ARHGEF1 Binding Mode: Challenges and Applications for Protein-Protein Interface Prediction. Frontiers in molecular biosciences, 8, 333.

Bouafia, A., Lofek, S., Bruneau, J., Chentout, L., Lamrini, H., Trinquand, A., ... & Kracker, S. (2019). Loss of ARHGEF1 causes a human primary antibody deficiency. The Journal of clinical investigation, 129(3), 1047-1060.

Shaifta, Y., MacKay, C. E., Irechukwu, N., O'Brien, K. A., Wright, D. B., Ward, J. P., & Knock, G. A. (2018). Transforming growth factor‐β enhances Rho‐kinase activity and contraction in airway smooth muscle via the nucleotide exchange factor ARHGEF1. The Journal of physiology, 596(1), 47-66.

MacKay, C. E., Shaifta, Y., Snetkov, V. V., Francois, A. A., Ward, J. P., & Knock, G. A. (2017). ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: role of Src-family kinases and ARHGEF1. Free Radical Biology and Medicine, 110, 316-331.

Toffali, L., Montresor, A., Mirenda, M., Scita, G., & Laudanna, C. (2017). SOS1, ARHGEF1, and DOCK2 rho-GEFs mediate JAK-dependent LFA-1 activation by chemokines. The Journal of Immunology, 198(2), 708-717.

Carbone, M. L., Chadeuf, G., Heurtebise-Chrétien, S., Prieur, X., Quillard, T., Goueffic, Y., ... & Loirand, G. (2017). Leukocyte RhoA exchange factor Arhgef1 mediates vascular inflammation and atherosclerosis. The Journal of clinical investigation, 127(12), 4516-4526.

Xiang, X., Zhuang, X., Li, S., & Shi, L. (2017). Arhgef1 is expressed in cortical neural progenitor cells and regulates neurite outgrowth of newly differentiated neurons. Neuroscience letters, 638, 27-34.

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For research use only. Not intended for any clinical use.

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