Human ARHGEF1 ELISA Kit (V2LY-0626-LY3705)

Name: Human ARHGEF1 ELISA Kit
SKU: V2LY-0626-LY3705
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0028 ng/mL

Detection Range

0.005-1 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

102.4 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 1

Function

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits (PubMed:9641915, PubMed:9641916).
Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase (PubMed:9641915, PubMed:9641916, PubMed:8810315, PubMed:30521495).
Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain (PubMed:9641916).
This GEF activity is inhibited by binding to activated GNA12 (PubMed:9641916).
Mediates angiotensin-2-induced RhoA activation (PubMed:20098430).

Biological Process

G protein-coupled receptor signaling pathway Source: Reactome
Positive regulation of apoptotic process Source: Reactome
Positive regulation of GTPase activity Source: GOC
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc

Cellular Location

Cytoplasm; Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.

Involvement in disease

Immunodeficiency 62 (IMD62): An autosomal recessive, primary immunologic disorder characterized by recurrent severe respiratory tract infections and bronchiectasis, due to antibody deficiency. Affected individuals have an abnormal B cell immunophenotype, with low levels of circulating memory B cells.

PTM

Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.

Gheyouche, E., Bagueneau, M., Loirand, G., Offmann, B., & Téletchéa, S. (2021). Structural Design and Analysis of the RHOA-ARHGEF1 Binding Mode: Challenges and Applications for Protein-Protein Interface Prediction. Frontiers in molecular biosciences, 8, 333.

Bouafia, A., Lofek, S., Bruneau, J., Chentout, L., Lamrini, H., Trinquand, A., ... & Kracker, S. (2019). Loss of ARHGEF1 causes a human primary antibody deficiency. The Journal of clinical investigation, 129(3), 1047-1060.

Shaifta, Y., MacKay, C. E., Irechukwu, N., O'Brien, K. A., Wright, D. B., Ward, J. P., & Knock, G. A. (2018). Transforming growth factor‐β enhances Rho‐kinase activity and contraction in airway smooth muscle via the nucleotide exchange factor ARHGEF1. The Journal of physiology, 596(1), 47-66.

MacKay, C. E., Shaifta, Y., Snetkov, V. V., Francois, A. A., Ward, J. P., & Knock, G. A. (2017). ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: role of Src-family kinases and ARHGEF1. Free Radical Biology and Medicine, 110, 316-331.

Toffali, L., Montresor, A., Mirenda, M., Scita, G., & Laudanna, C. (2017). SOS1, ARHGEF1, and DOCK2 rho-GEFs mediate JAK-dependent LFA-1 activation by chemokines. The Journal of Immunology, 198(2), 708-717.

Carbone, M. L., Chadeuf, G., Heurtebise-Chrétien, S., Prieur, X., Quillard, T., Goueffic, Y., ... & Loirand, G. (2017). Leukocyte RhoA exchange factor Arhgef1 mediates vascular inflammation and atherosclerosis. The Journal of clinical investigation, 127(12), 4516-4526.

Xiang, X., Zhuang, X., Li, S., & Shi, L. (2017). Arhgef1 is expressed in cortical neural progenitor cells and regulates neurite outgrowth of newly differentiated neurons. Neuroscience letters, 638, 27-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ARHGEF1 Matched Antibody Pair (104) (CAT#: APMAB-104LY)

Antibody Pairs

ARHGEF1 Matched Antibody Pair (104) (CAT#: APMAB-104LY)

Related Products

ARHGEF1 Matched Antibody Pair (104) (CAT#: APMAB-104LY)

Mouse Anti-ARHGEF1 Recombinant Antibody (4C4) (CAT#: CBMAB-A0465-LY)

Mouse Anti-ARHGEF1 Recombinant Antibody (EG234) (CAT#: CBMAB-EN261-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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