Mouse Anti-ARHGEF1 Recombinant Antibody (EG234) (CBMAB-EN261-LY)

Name: Mouse Anti-ARHGEF1 Recombinant Antibody (EG234)
SKU: CBMAB-EN261-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARHGEF1 Recombinant Antibody (EG234) CBMAB-EN261-LY in WB and IP

Growth-arrested HASMCs were treated with MCP1 (50 ng/ml) in the presence or absence of CCR2A (25 nm) or Pertussis toxin (PT) (50 ng/ml) for 10 min or left untreated, and cell extracts were prepared. Equal amounts of protein from the control and each treatment were immunoprecipitated (IP) with phosphotyrosine antibodies, and the immunocomplexes were immunoblotted (IB) with anti-p115 RhoGEF antibodies or analyzed by pulldown assay for Rac1 activation using GST-PAK1 beads. ...View More

Singh, N. K., Janjanam, J., & Rao, G. N. (2017). p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation. Journal of Biological Chemistry, 292(34), 14080-14091.

Mouse Anti-ARHGEF1 Recombinant Antibody (EG234) CBMAB-EN261-LY in IF

HASMCs were transfected with control or p115 RhoGEF siRNA, quiesced, treated with and without MCP1 (50 ng/ml) for 30 min, fixed, permeabilized, and immunostained for NFATc1 using its specific antibody, followed by probing with Alexa Fluor 488-conjugated secondary antibody. The images were captured under a Zeiss Oberver.Z1 fluorescence microscope using an AxioCam MRm camera. ...View More

Mouse Anti-ARHGEF1 Recombinant Antibody (EG234) CBMAB-EN261-LY in IHC

Arteries from 2 weeks post-BI rats that were administered 10 μg of control or p115 RhoGEF siRNA were isolated and fixed. Cross-sections were made and stained with H&E, and the I/M ratios were calculated. Left panel, representative pictures of BI common carotid artery cross-sections that were stained with H&E. Right panel, the bar graph shows mean ± S.D. values of the I/M ratios of the injured common carotid arteries from six animals. *, p < 0.05 versus uninjured or siControl + BI. ...View More

Datasheet

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Basic Information

Host Animal

Mouse

Clone

EG234

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 748-912 mapping at the C-terminus of RhoGEF p115 of human origin.

Specificity

Human

Antibody Isotype

IgG2b, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 1

Introduction

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	9138
Mouse	16801
Rat	60323

UniProt ID

Human	Q92888
Mouse	Q61210
Rat	Q9Z1I6

Alternative Names

Rho Guanine Nucleotide Exchange Factor 1; Rho Guanine Nucleotide Exchange Factor (GEF) 1; 115 KDa Guanine Nucleotide Exchange Factor; P115-RHOGEF; P115RhoGEF; SUB1.5;

Function

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits (PubMed:9641915, PubMed:9641916).
Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase (PubMed:9641915, PubMed:9641916, PubMed:8810315, PubMed:30521495).
Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain (PubMed:9641916).
This GEF activity is inhibited by binding to activated GNA12 (PubMed:9641916).
Mediates angiotensin-2-induced RhoA activation (PubMed:20098430).

Biological Process

G protein-coupled receptor signaling pathway Source: Reactome
Positive regulation of apoptotic process Source: Reactome
Positive regulation of GTPase activity Source: GOC
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc

Cellular Location

Cytoplasm; Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.

Involvement in disease

Immunodeficiency 62 (IMD62): An autosomal recessive, primary immunologic disorder characterized by recurrent severe respiratory tract infections and bronchiectasis, due to antibody deficiency. Affected individuals have an abnormal B cell immunophenotype, with low levels of circulating memory B cells.

PTM

Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.

Gheyouche, E., Bagueneau, M., Loirand, G., Offmann, B., & Téletchéa, S. (2021). Structural Design and Analysis of the RHOA-ARHGEF1 Binding Mode: Challenges and Applications for Protein-Protein Interface Prediction. Frontiers in molecular biosciences, 8, 333.

Bouafia, A., Lofek, S., Bruneau, J., Chentout, L., Lamrini, H., Trinquand, A., ... & Kracker, S. (2019). Loss of ARHGEF1 causes a human primary antibody deficiency. The Journal of clinical investigation, 129(3), 1047-1060.

Shaifta, Y., MacKay, C. E., Irechukwu, N., O'Brien, K. A., Wright, D. B., Ward, J. P., & Knock, G. A. (2018). Transforming growth factor‐β enhances Rho‐kinase activity and contraction in airway smooth muscle via the nucleotide exchange factor ARHGEF1. The Journal of physiology, 596(1), 47-66.

MacKay, C. E., Shaifta, Y., Snetkov, V. V., Francois, A. A., Ward, J. P., & Knock, G. A. (2017). ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: role of Src-family kinases and ARHGEF1. Free Radical Biology and Medicine, 110, 316-331.

Toffali, L., Montresor, A., Mirenda, M., Scita, G., & Laudanna, C. (2017). SOS1, ARHGEF1, and DOCK2 rho-GEFs mediate JAK-dependent LFA-1 activation by chemokines. The Journal of Immunology, 198(2), 708-717.

Carbone, M. L., Chadeuf, G., Heurtebise-Chrétien, S., Prieur, X., Quillard, T., Goueffic, Y., ... & Loirand, G. (2017). Leukocyte RhoA exchange factor Arhgef1 mediates vascular inflammation and atherosclerosis. The Journal of clinical investigation, 127(12), 4516-4526.

Xiang, X., Zhuang, X., Li, S., & Shi, L. (2017). Arhgef1 is expressed in cortical neural progenitor cells and regulates neurite outgrowth of newly differentiated neurons. Neuroscience letters, 638, 27-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ARHGEF1 Matched Antibody Pair (104) (CAT#: APMAB-104LY)

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For research use only. Not intended for any clinical use.

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