Rabbit Anti-ARHGEF6 Recombinant Antibody (C23D2) (CBMAB-CP0347-LY)

The product is antibody recognizes ARHGEF6. The antibody C23D2 immunoassay techniques such as: WB,IF (ICC).
See all ARHGEF6 antibodies

Published Data

Rabbit Anti-ARHGEF6 Recombinant Antibody (C23D2) CBMAB-CP0347-LY in Immunoblot

Immunoblotting analysis of GST-Pdlim5 pull-down assay. Eluates were subjected to immunoblotting with anti-Arhgef6 antibody. Coomassie staining demonstrates equal loading of GST-Pdlim5 proteins. ...View More

Yan, Y., Tsukamoto, O., Nakano, A., Kato, H., Kioka, H., Ito, N., ... & Takashima, S. (2015). Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5. Nature communications, 6(1), 6137.

Rabbit Anti-ARHGEF6 Recombinant Antibody (C23D2) CBMAB-CP0347-LY in WB

Western blots labeled with antibodies against Cool2/αPix. ...View More

Sabirzhanova, I., Liu, C., Zhao, J., Bramlett, H., Dietrich, W. D., & Hu, B. (2013). Changes in the GEF-H1 pathways after traumatic brain injury. Journal of neurotrauma, 30(16), 1449-1456.

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

C23D2

Antibody Isotype

IgG

Application

WB, IF

Basic Information

Immunogen

Synthetic peptide corresponding to residues near the carboxy terminus of human Cool2/αPix.

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IF(ICC)	1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Rac/Cdc42 Guanine Nucleotide Exchange Factor 6

Introduction

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific cognitive disability. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	9459
Mouse	73341
Rat	363509
Dog	610290

UniProt ID

Human	Q15052
Mouse	Q8K4I3
Rat	Q5XXR3
Dog	E2R0Z1

Alternative Names

Rac/Cdc42 Guanine Nucleotide Exchange Factor 6; Rac/Cdc42 Guanine Nucleotide Exchange Factor (GEF) 6; Rac/Cdc42 Guanine Exchange Factor (GEF) 6; Rho Guanine Nucleotide Exchange Factor 6; PAK-Interacting Exchange Factor; Alpha; Alpha-PIX; Cool-2; COOL2;

Function

Acts as a RAC1 guanine nucleotide exchange factor (GEF).

Biological Process

Apoptotic process Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
JNK cascade Source: UniProtKB
Lamellipodium assembly Source: UniProtKB
Positive regulation of apoptotic process Source: Reactome
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Lamellipodium

References

Mamula, D., Korthals, M., Hradsky, J., Gottfried, A., Fischer, K. D., & Tedford, K. (2021). Arhgef6 (alpha‐PIX) cytoskeletal regulator signals to GTPases and Cofilin to couple T cell migration speed and persistence. Journal of Leukocyte Biology.

Khimsuriya, Y. M., & Chauhan, J. B. (2018). Pathogenic predictions of non-synonymous variants and their impacts: A computational assessment of ARHGEF6 gene. Egyptian Journal of Medical Human Genetics, 19(4), 333-344.

Pratt-Hyatt, M., Haeusler, R. A., Yuen, K. C., & Shaw, W. (2018). A Single Nucleotide Polymorphism in the ARHGEF6 Gene is Associated with Increased Risk for Autism Spectrum Disorder. Hereditary Genetics, 7(2), 117-127.

Zhu, C., Cheng, C., Wang, Y., Muhammad, W., Liu, S., Zhu, W., ... & Chai, R. (2018). Loss of ARHGEF6 causes hair cell stereocilia deficits and hearing loss in mice. Frontiers in molecular neuroscience, 11, 362.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

ARHGEF6 Matched Antibody Pair (106)

Related Products

Mouse Anti-ARHGEF6 Recombinant Antibody (2E5)

ARHGEF6 Matched Antibody Pair (106)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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