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Mouse Anti-ASF1A Antibody (2A5F8) (CBMAB-0134-YC)

Provided herein are mouse monoclonal antibodies against Human ASF1A. The antibody clone 1H3 can be used for immunoassay techniques, such as IP and MA.
See all ASF1A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2A5F8
Antibody Isotype
IgG1
Application
ELISA, WB, FC

Basic Information

Immunogen
Purified recombinant fragment of human ASF1A (AA: 1-204) expressed in E. Coli.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:2,000
WB1:100-1:2,000
FC1:10-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anti-Silencing Function 1A Histone Chaperone
Introduction
Histone chaperone ASF1A is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.
Entrez Gene ID
Human25842
Mouse66403
Rat294408
Frog100490579
Zebrafish792029
UniProt ID
HumanP50553
MouseQ02067
RatP19359
FrogQ69DB9
Alternative Names
CIA; DKFZP547E2110
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Biological Process
DNA repair Source: MGI
DNA replication-dependent nucleosome assembly Source: UniProtKB
DNA replication-independent nucleosome assembly Source: UniProtKB
Muscle cell differentiation Source: Ensembl
Negative regulation of chromatin silencing Source: UniProtKB
Nucleosome assembly Source: MGI
Osteoblast differentiation Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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