Mouse Anti-ASF1A Recombinant Antibody (CBT4630) (V2LY-0625-LY804)

Name: Mouse Anti-ASF1A Recombinant Antibody (CBT4630)
SKU: V2LY-0625-LY804
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ASF1A Recombinant Antibody (CBT4630) (V2LY-0625-LY804) in ELISA

Mouse Anti-ASF1A Recombinant Antibody (CBT4630) (V2LY-0625-LY804) in WB

Mouse Anti-ASF1A Recombinant Antibody (CBT4630) (V2LY-0625-LY804) in FC

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4630

Application

Immunogen

Purified recombinant fragment of human ASF1A (AA: 1-204) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Anti-Silencing Function 1A Histone Chaperone

Entrez Gene ID

25842

UniProt ID

Q9Y294

Function

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Biological Process

DNA repair Source: MGI
DNA replication-dependent nucleosome assembly Source: UniProtKB
DNA replication-independent nucleosome assembly Source: UniProtKB
Muscle cell differentiation Source: Ensembl
Negative regulation of chromatin silencing Source: UniProtKB
Nucleosome assembly Source: MGI
Osteoblast differentiation Source: Ensembl

Cellular Location

Nucleus

PTM

Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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