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Rabbit Anti-ASF1A Recombinant Antibody (C6E10) (CBMAB-A3754-YC)

Provided herein is a Rabbit monoclonal antibody against Human Anti-Silencing Function 1A Histone Chaperone. The antibody can be used for immunoassay techniques, such as WB, IP, IHC-P, IF.
See all ASF1A antibodies
Published Data
Rabbit Anti-ASF1A Recombinant Antibody (C6E10) CBMAB-A3754-YC in IF and Immunoblot
Ectopic expression of ASF1A in HT29 cells verified using immunoblotting. HT29 cells were infected with control- and ASF1A-lentiviral vectors and over-expression of ASF1A was revealed by immunoblotting (Left panel). Immuno-fluorescence (IF) staining shows enhanced histone H3-K56 acetylation in HT29 cells expressing ectopic ASF1A (Right panel). ...View More
Liang, X., Yuan, X., Yu, J., Wu, Y., Li, K., Sun, C., ... & Xu, D. (2017). Histone chaperone ASF1A predicts poor outcomes for patients with gastrointestinal cancer and drives cancer progression by stimulating transcription of β-catenin target genes. EBioMedicine, 21, 104-116.
Rabbit Anti-ASF1A Recombinant Antibody (C6E10) CBMAB-A3754-YC in IHC
Cell morphological assays assessed by May-Grunwald Giemsa staining of K562 and MEG01 cells expressing either empty vector (shNC) or shASF1A-1/shASF1A-2. Arrows indicate cells with matured morphology, which exhibit kidney-shape nucleus and decreased nuclear/cytoplasm ratio. Statistical significance was determined by Student's t test. Data are shown as mean ± standard deviation (SD). ...View More
Yin, X., Zhou, M., Zhang, L., Fu, Y., Xu, M., Wang, X., ... & Chen, C. (2022). Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling. Cell Death & Disease, 13(10), 842.
Rabbit Anti-ASF1A Recombinant Antibody (C6E10) CBMAB-A3754-YC in WB
Western blot analysis of ASF1A in K562 and MEG01 cells pretreated with solvent (control) or DMSO/ATRA/PMA. Statistical significance was determined by Student's t-test. ...View More
Yin, X., Zhou, M., Zhang, L., Fu, Y., Xu, M., Wang, X., ... & Chen, C. (2022). Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling. Cell Death & Disease, 13(10), 842.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Monkey
Clone
C6E10
Antibody Isotype
IgG
Application
WB, IP, IHC-P, IF

Basic Information

Immunogen
Synthetic peptide corresponding to the carboxy terminus of the human ASF1A protein.
Specificity
Human, Mouse, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IF(ICC)1:100
IHC-P1:800
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anti-Silencing Function 1A Histone Chaperone
Introduction
ASF1A is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones
Entrez Gene ID
Human25842
Mouse66403
Cattle618099
Monkey714761
Chicken723780
UniProt ID
HumanP50553
MouseQ02067
CattleQ2KIG1
MonkeyA0A2K5VAE3
ChickenP79987
Alternative Names
Anti-Silencing Function 1A Histone Chaperone; Anti-Silencing Function Protein 1 Homolog A; CCG1-Interacting Factor A; HAsf1a; HAsf1; HCIA; CIA;
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Biological Process
DNA repair Source: MGI
DNA replication-dependent nucleosome assembly Source: UniProtKB
DNA replication-independent nucleosome assembly Source: UniProtKB
Muscle cell differentiation Source: Ensembl
Negative regulation of chromatin silencing Source: UniProtKB
Nucleosome assembly Source: MGI
Osteoblast differentiation Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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