Mouse Anti-ATG4A Recombinant Antibody (CBT3279) (V2LY-0625-LY1044)

Name: Mouse Anti-ATG4A Recombinant Antibody (CBT3279)
SKU: V2LY-0625-LY1044
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT3279

Antibody Isotype

IgG2b

Application

ICC, FC

Basic Information

Immunogen

Purified recombinant fragment of human ATG4A (AA: 258-398) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:100-1:400
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Autophagy Related 4A Cysteine Peptidase

Entrez Gene ID

115201

UniProt ID

Q8WYN0

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Biological Process

Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB

Cellular Location

Cytoplasm

More Infomation

References

Umesh, Prerna, K., & Dubey, V. K. (2020). Virtual screening and repurposing of FDA-approved drugs from ZINC database to identify potential autophagy inhibitors exploiting autophagy related 4A cysteine peptidase as a target: potential as novel anti-cancer molecule. Journal of Biomolecular Structure and Dynamics, 1-17.

Yan, C., Zhao, J., Qin, Y., Zhao, F., Ji, L., & Zhang, J. (2020). Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways. Molecular Medicine Reports, 22(2), 1295-1302.

Hait, A. S., Olagnier, D., Sancho-Shimizu, V., Skipper, K. A., Helleberg, M., Larsen, S. M., ... & Mogensen, T. H. (2020). Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans. Science immunology, 5(54), eabc2691.

Guo, L., Zhou, L., Gao, Q., Zhang, A., Wei, J., Hong, D., ... & Xu, G. (2017). MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guerin infection by targeting ATG4a in RAW264. 7 macrophage cells. PLoS One, 12(6), e0179772.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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