Sign in or Register   Sign in or Register
  |  

Mouse Anti-ATG4A Recombinant Antibody (CBYC-A853) (CBMAB-A3926-YC)

Provided herein is a Rabbit monoclonal antibody against Human Autophagy Related 4A Cysteine Peptidase. The antibody can be used for immunoassay techniques, such as IHC-P, IP, WB.
See all ATG4A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A853
Antibody Isotype
IgG2b
Application
ELISA, IF, WB, FC

Basic Information

Immunogen
Purified recombinant fragment of human ATG4A (aa: 258-398) expressed in E. Coli.
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
IF(ICC)1:100-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 4A Cysteine Peptidase
Introduction
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, a
Entrez Gene ID
Human115201
Mouse666468
Rat678769
UniProt ID
HumanQ8WYN0
MouseQ8C9S8
RatB1H274
Alternative Names
Autophagy Related 4A Cysteine Peptidase; Autophagy-Related Cysteine Endopeptidase 2; Autophagy-Related Protein 4 Homolog A; HAPG4A; APG4A; AUTL2; AUT-Like 2, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae); APG4
Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Biological Process
Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB
Cellular Location
Cytoplasm
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ATG4A Recombinant Antibody (CBYC-A853)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare