Mouse Anti-ATG4A Recombinant Antibody (CBYC-A854) (CBMAB-A3927-YC)

Provided herein is a Mouse monoclonal antibody against Human Autophagy Related 4A Cysteine Peptidase. The antibody can be used for immunoassay techniques, such as ICC, IF, IP, WB.
See all ATG4A antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

CBYC-A854

Antibody Isotype

IgG2b

Application

ICC, IF, IP, WB

Basic Information

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A isoform 1 Glu2-Val398.

Specificity

Human, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
IP	25 µg/ml
IF(ICC)	8-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Autophagy Related 4A Cysteine Peptidase

Introduction

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, a

Entrez Gene ID

Human	115201
Mouse	666468

UniProt ID

Human	Q8WYN0
Mouse	Q8C9S8

Alternative Names

Autophagy Related 4A Cysteine Peptidase; Autophagy-Related Cysteine Endopeptidase 2; Autophagy-Related Protein 4 Homolog A; HAPG4A; APG4A; AUTL2; AUT-Like 2, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae); APG4

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Biological Process

Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB

Cellular Location

Cytoplasm

References

Umesh, Prerna, K., & Dubey, V. K. (2020). Virtual screening and repurposing of FDA-approved drugs from ZINC database to identify potential autophagy inhibitors exploiting autophagy related 4A cysteine peptidase as a target: potential as novel anti-cancer molecule. Journal of Biomolecular Structure and Dynamics, 1-17.

Yan, C., Zhao, J., Qin, Y., Zhao, F., Ji, L., & Zhang, J. (2020). Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways. Molecular Medicine Reports, 22(2), 1295-1302.

Hait, A. S., Olagnier, D., Sancho-Shimizu, V., Skipper, K. A., Helleberg, M., Larsen, S. M., ... & Mogensen, T. H. (2020). Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans. Science immunology, 5(54), eabc2691.

Guo, L., Zhou, L., Gao, Q., Zhang, A., Wei, J., Hong, D., ... & Xu, G. (2017). MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guerin infection by targeting ATG4a in RAW264. 7 macrophage cells. PLoS One, 12(6), e0179772.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ATG4A Recombinant Antibody (CBYC-A853)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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