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Mouse Anti-ATG4B Recombinant Antibody (CBT2850) (V2LY-0625-LY1290)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2850
Antibody Isotype
IgG1
Application
WB, IHC, FC

Basic Information

Immunogen
Purified recombinant fragment of human ATG4B (AA: 1-221) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Autophagy Related 4B Cysteine Peptidase
Entrez Gene ID
23192
UniProt ID
Q9Y4P1
Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Biological Process
Autophagy Source: BHF-UCL
Macroautophagy Source: Reactome
Positive regulation of protein catabolic process Source: Ensembl
Protein delipidation Source: MGI
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB
Cellular Location
Cytoplasm
More Infomation

Wu, Y., Mao, Q., & Liang, X. (2021). Targeting the MicroRNA-490-3p-ATG4B-Autophagy Axis Relieves Myocardial Injury in Ischemia Reperfusion. Journal of Cardiovascular Translational Research, 14, 173-183.

Bortnik, S., Tessier-Cloutier, B., Leung, S., Xu, J., Asleh, K., Burugu, S., ... & Gorski, S. M. (2020). Differential expression and prognostic relevance of autophagy-related markers ATG4B, GABARAP, and LC3B in breast cancer. Breast Cancer Research and Treatment, 183(3), 525-547.

Shin, J. H., Park, S. J., Jo, D. S., Park, N. Y., Kim, J. B., Bae, J. E., ... & Cho, D. H. (2019). Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy, 15(9), 1495-1505.

Chu, J., Fu, Y., Xu, J., Zheng, X., Gu, Q., Luo, X., ... & Li, M. (2018). ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Archives of biochemistry and biophysics, 644, 29-36.

Bosc, D., Vezenkov, L., Bortnik, S., An, J., Xu, J., Choutka, C., ... & Young, R. N. (2018). A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B. Scientific reports, 8(1), 1-17.

Zhang, N., Wu, Y., Lyu, X., Li, B., Yan, X., Xiong, H., ... & He, F. (2017). HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells. Cancer letters, 409, 81-90.

Wu, Y., Dai, X., Ni, Z., Yan, X., He, F., & Lian, J. (2017). The downregulation of ATG4B mediated by microRNA-34a/34c-5p suppresses rapamycin-induced autophagy. Iranian journal of basic medical sciences, 20(10), 1125.

Pengo, N., Agrotis, A., Prak, K., Jones, J., & Ketteler, R. (2017). A reversible phospho-switch mediated by ULK1 regulates the activity of autophagy protease ATG4B. Nature communications, 8(1), 1-10.

An, Z., Qiao, F., Lu, Q., Ma, Y., Liu, Y., Lu, F., & Xu, Z. (2017). Interleukin-6 downregulated vascular smooth muscle cell contractile proteins via ATG4B-mediated autophagy in thoracic aortic dissection. Heart and vessels, 32(12), 1523-1535.

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For research use only. Not intended for any clinical use.

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