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Mouse Anti-ATG4B Recombinant Antibody (EG293) (CBMAB-EN322-LY)

The product is antibody recognizes ATG4B . The antibody EG293 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000.
See all ATG4B antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
EG293
Antibody Isotype
IgG2a
Application
WB, IHC-P, IF

Basic Information

Immunogen
ATG4B fusion protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:400
IHC-P1:500
WB1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Autophagy Related 4B Cysteine Peptidase
Introduction
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human23192
Mouse66615
UniProt ID
HumanQ9Y4P1
MouseQ8BGE6
Alternative Names
Autophagy Related 4B Cysteine Peptidase; Autophagy-Related Cysteine Endopeptidase 1; Autophagy-Related Protein 4 Homolog B; AUT-Like 1 Cysteine Endopeptidase; Autophagin-1; HAPG4B; APG4B; AUTL1; ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae);
Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Biological Process
Autophagy Source: BHF-UCL
Macroautophagy Source: Reactome
Positive regulation of protein catabolic process Source: Ensembl
Protein delipidation Source: MGI
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB
Cellular Location
Cytoplasm

Wu, Y., Mao, Q., & Liang, X. (2021). Targeting the MicroRNA-490-3p-ATG4B-Autophagy Axis Relieves Myocardial Injury in Ischemia Reperfusion. Journal of Cardiovascular Translational Research, 14, 173-183.

Bortnik, S., Tessier-Cloutier, B., Leung, S., Xu, J., Asleh, K., Burugu, S., ... & Gorski, S. M. (2020). Differential expression and prognostic relevance of autophagy-related markers ATG4B, GABARAP, and LC3B in breast cancer. Breast Cancer Research and Treatment, 183(3), 525-547.

Shin, J. H., Park, S. J., Jo, D. S., Park, N. Y., Kim, J. B., Bae, J. E., ... & Cho, D. H. (2019). Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy, 15(9), 1495-1505.

Chu, J., Fu, Y., Xu, J., Zheng, X., Gu, Q., Luo, X., ... & Li, M. (2018). ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Archives of biochemistry and biophysics, 644, 29-36.

Bosc, D., Vezenkov, L., Bortnik, S., An, J., Xu, J., Choutka, C., ... & Young, R. N. (2018). A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B. Scientific reports, 8(1), 1-17.

Zhang, N., Wu, Y., Lyu, X., Li, B., Yan, X., Xiong, H., ... & He, F. (2017). HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells. Cancer letters, 409, 81-90.

Wu, Y., Dai, X., Ni, Z., Yan, X., He, F., & Lian, J. (2017). The downregulation of ATG4B mediated by microRNA-34a/34c-5p suppresses rapamycin-induced autophagy. Iranian journal of basic medical sciences, 20(10), 1125.

Pengo, N., Agrotis, A., Prak, K., Jones, J., & Ketteler, R. (2017). A reversible phospho-switch mediated by ULK1 regulates the activity of autophagy protease ATG4B. Nature communications, 8(1), 1-10.

An, Z., Qiao, F., Lu, Q., Ma, Y., Liu, Y., Lu, F., & Xu, Z. (2017). Interleukin-6 downregulated vascular smooth muscle cell contractile proteins via ATG4B-mediated autophagy in thoracic aortic dissection. Heart and vessels, 32(12), 1523-1535.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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