Human Recombinant ATG4B protein, His Tag (V2LY-0526-LY2195)

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Basic Information

Expressed Host
E. coli
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant ATG4B protein, His Tag consist of Amino Acid: Full Length and predicts a molecular mass of 52 kDa.
Molecule Mass
52 kDa
Sequence
Amino Acid: Full Length
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
Batch dependent.
Endotoxin
Please contact us for more information.
Format
Liquid
Buffer
PBS
Preservative
None
Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
More Infomation

Target

Full Name
Autophagy Related 4B Cysteine Peptidase
Function
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Biological Process
Autophagy Source: BHF-UCL
Macroautophagy Source: Reactome
Positive regulation of protein catabolic process Source: Ensembl
Protein delipidation Source: MGI
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB
Cellular Location
Cytoplasm

Wu, Y., Mao, Q., & Liang, X. (2021). Targeting the MicroRNA-490-3p-ATG4B-Autophagy Axis Relieves Myocardial Injury in Ischemia Reperfusion. Journal of Cardiovascular Translational Research, 14, 173-183.

Bortnik, S., Tessier-Cloutier, B., Leung, S., Xu, J., Asleh, K., Burugu, S., ... & Gorski, S. M. (2020). Differential expression and prognostic relevance of autophagy-related markers ATG4B, GABARAP, and LC3B in breast cancer. Breast Cancer Research and Treatment, 183(3), 525-547.

Shin, J. H., Park, S. J., Jo, D. S., Park, N. Y., Kim, J. B., Bae, J. E., ... & Cho, D. H. (2019). Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy, 15(9), 1495-1505.

Chu, J., Fu, Y., Xu, J., Zheng, X., Gu, Q., Luo, X., ... & Li, M. (2018). ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Archives of biochemistry and biophysics, 644, 29-36.

Bosc, D., Vezenkov, L., Bortnik, S., An, J., Xu, J., Choutka, C., ... & Young, R. N. (2018). A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B. Scientific reports, 8(1), 1-17.

Zhang, N., Wu, Y., Lyu, X., Li, B., Yan, X., Xiong, H., ... & He, F. (2017). HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells. Cancer letters, 409, 81-90.

Wu, Y., Dai, X., Ni, Z., Yan, X., He, F., & Lian, J. (2017). The downregulation of ATG4B mediated by microRNA-34a/34c-5p suppresses rapamycin-induced autophagy. Iranian journal of basic medical sciences, 20(10), 1125.

Pengo, N., Agrotis, A., Prak, K., Jones, J., & Ketteler, R. (2017). A reversible phospho-switch mediated by ULK1 regulates the activity of autophagy protease ATG4B. Nature communications, 8(1), 1-10.

An, Z., Qiao, F., Lu, Q., Ma, Y., Liu, Y., Lu, F., & Xu, Z. (2017). Interleukin-6 downregulated vascular smooth muscle cell contractile proteins via ATG4B-mediated autophagy in thoracic aortic dissection. Heart and vessels, 32(12), 1523-1535.

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For research use only. Not intended for any clinical use.

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