Mouse Anti-ATG9A Recombinant Antibody (F3) (CBMAB-A3954-YC)

Name: Mouse Anti-ATG9A Recombinant Antibody (F3)
SKU: CBMAB-A3954-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

IP, WB, IF, ELISA

Immunogen

Amino acids 561-839 mapping at the C-terminus of Atg9a of human origin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Autophagy Related 9A

Introduction

ATG9A is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. ATG9A plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering

Entrez Gene ID

Human	79065
Mouse	245860
Rat	363254

UniProt ID

Human	Q7Z3C6
Mouse	Q68FE2
Rat	Q5FWU3

Alternative Names

Autophagy Related 9A; APG9-Like 1; APG9L1; MATG9; ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae); APG9 Autophagy 9-Like 1 (S. Cerevisiae);

Function

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Biological Process

Autophagosome assembly Source: MGI
Autophagy of mitochondrion Source: GO_Central
Late nucleophagy Source: GO_Central
Protein localization to phagophore assembly site Source: GO_Central
Protein transport Source: UniProtKB-KW

Cellular Location

Late endosome membrane; Trans-Golgi network membrane; Endoplasmic reticulum membrane; Autophagosome membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.

Topology

Cytoplasmic: 2-66 aa
Helical: 67-87 aa
Lumenal: 88-131 aa
Helical: 132-152 aa
Cytoplasmic: 153-289 aa
Helical: 290-310 aa
Lumenal: 311-371 aa
Helical: 372-392 aa
Cytoplasmic: 393-400 aa
Helical: 401-421 aa
Lumenal: 422-473 aa
Helical: 474-494 aa
Cytoplasmic: 495-839 aa

Kimkong, I., & Kunanopparat, A. (2020). Autophagy related protein 9A increase in hepatitis B virus-associated hepatocellular carcinoma and the role in apoptosis. World Journal of Hepatology, 12(12), 1367.

Mailler, E., Waheed, A. A., Park, S. Y., Gershlick, D. C., Freed, E. O., & Bonifacino, J. S. (2019). The autophagy protein ATG9A promotes HIV-1 infectivity. Retrovirology, 16(1), 1-20.

Kong, Y., Huang, T., Zhang, H., Zhang, Q., Ren, J., Guo, X., ... & Liu, L. (2019). The lncRNA NEAT1/miR-29b/Atg9a axis regulates IGFBPrP1-induced autophagy and activation of mouse hepatic stellate cells. Life sciences, 237, 116902.

Fan, H., Yuan, F., Yun, Y., Wu, T., Lu, L., Liu, J., ... & Chen, S. Y. (2019). MicroRNA-34a mediates ethanol-induced impairment of neural differentiation of neural crest cells by targeting autophagy-related gene 9a. Experimental neurology, 320, 112981.

Davies, A. K., Itzhak, D. N., Edgar, J. R., Archuleta, T. L., Hirst, J., Jackson, L. P., ... & Borner, G. H. (2018). AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Nature communications, 9(1), 1-21.

Jiang, C. Y., Yang, B. Y., Zhao, S., Shao, S. H., Bei, X. Y., Shi, F., ... & Ruan, Y. (2018). Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression. Cell death & disease, 9(4), 1-12.

Pang, J., Xiong, H., Lin, P., Lai, L., Yang, H., Liu, Y., ... & Zheng, Y. (2017). Activation of miR-34a impairs autophagic flux and promotes cochlear cell death via repressing ATG9A: implications for age-related hearing loss. Cell death & disease, 8(10), e3079-e3079.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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