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Mouse Anti-ATG9A Recombinant Antibody (F3) (CBMAB-A3954-YC)

Provided herein is a Hamster monoclonal antibody against Human Autophagy Related 9A. The antibody can be used for immunoassay techniques, such as WB, ELISA, IHC.
See all ATG9A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
F3
Antibody Isotype
IgG1, κ
Application
IP, WB, IF, ELISA

Basic Information

Immunogen
Amino acids 561-839 mapping at the C-terminus of Atg9a of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 9A
Introduction
ATG9A is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. ATG9A plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering
Entrez Gene ID
Human79065
Mouse245860
Rat363254
UniProt ID
HumanQ7Z3C6
MouseQ68FE2
RatQ5FWU3
Alternative Names
Autophagy Related 9A; APG9-Like 1; APG9L1; MATG9; ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae); APG9 Autophagy 9-Like 1 (S. Cerevisiae);
Function
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Biological Process
Autophagosome assembly Source: MGI
Autophagy of mitochondrion Source: GO_Central
Late nucleophagy Source: GO_Central
Protein localization to phagophore assembly site Source: GO_Central
Protein transport Source: UniProtKB-KW
Cellular Location
Late endosome membrane; Trans-Golgi network membrane; Endoplasmic reticulum membrane; Autophagosome membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
Topology
Cytoplasmic: 2-66 aa
Helical: 67-87 aa
Lumenal: 88-131 aa
Helical: 132-152 aa
Cytoplasmic: 153-289 aa
Helical: 290-310 aa
Lumenal: 311-371 aa
Helical: 372-392 aa
Cytoplasmic: 393-400 aa
Helical: 401-421 aa
Lumenal: 422-473 aa
Helical: 474-494 aa
Cytoplasmic: 495-839 aa

Kimkong, I., & Kunanopparat, A. (2020). Autophagy related protein 9A increase in hepatitis B virus-associated hepatocellular carcinoma and the role in apoptosis. World Journal of Hepatology, 12(12), 1367.

Mailler, E., Waheed, A. A., Park, S. Y., Gershlick, D. C., Freed, E. O., & Bonifacino, J. S. (2019). The autophagy protein ATG9A promotes HIV-1 infectivity. Retrovirology, 16(1), 1-20.

Kong, Y., Huang, T., Zhang, H., Zhang, Q., Ren, J., Guo, X., ... & Liu, L. (2019). The lncRNA NEAT1/miR-29b/Atg9a axis regulates IGFBPrP1-induced autophagy and activation of mouse hepatic stellate cells. Life sciences, 237, 116902.

Fan, H., Yuan, F., Yun, Y., Wu, T., Lu, L., Liu, J., ... & Chen, S. Y. (2019). MicroRNA-34a mediates ethanol-induced impairment of neural differentiation of neural crest cells by targeting autophagy-related gene 9a. Experimental neurology, 320, 112981.

Davies, A. K., Itzhak, D. N., Edgar, J. R., Archuleta, T. L., Hirst, J., Jackson, L. P., ... & Borner, G. H. (2018). AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Nature communications, 9(1), 1-21.

Jiang, C. Y., Yang, B. Y., Zhao, S., Shao, S. H., Bei, X. Y., Shi, F., ... & Ruan, Y. (2018). Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression. Cell death & disease, 9(4), 1-12.

Pang, J., Xiong, H., Lin, P., Lai, L., Yang, H., Liu, Y., ... & Zheng, Y. (2017). Activation of miR-34a impairs autophagic flux and promotes cochlear cell death via repressing ATG9A: implications for age-related hearing loss. Cell death & disease, 8(10), e3079-e3079.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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