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Rabbit Anti-ATG9A Recombinant Antibody (BA0202) (CBMAB-0111CQ)

This product is a rabbit antibody that recognizes ATG9A. The antibody BA0202 can be used for immunoassay techniques such as: FC, ICC, IHC-P, IP, WB.
See all ATG9A antibodies
Published Data
Rabbit Anti-ATG9A Recombinant Antibody (BA0202) (CBMAB-0111CQ) in IF
Immunofluorescence analysis was performed to evaluate the expression levels and cellular localization of Atg9 (original magnification × 20). ...View More
Barbiera, A., Sorrentino, S., Lepore, E., Carfì, A., Sica, G., Dobrowolny, G., & Scicchitano, B. M. (2020). Taurine attenuates catabolic processes related to the onset of sarcopenia. International journal of molecular sciences, 21(22), 8865.
Rabbit Anti-ATG9A Recombinant Antibody (BA0202) (CBMAB-0111CQ) in IF
HEK293 cells were either fed or starved for 1 h, immunolabelled for ATG13 and ATG9 and imaged by wide-field microscopy. Arrowheads in inserts point at ATG13 particles associating with ATG9. Bar corresponds to 10 mm. ...View More
Karanasios, E., Walker, S. A., Okkenhaug, H., Manifava, M., Hummel, E., Zimmermann, H., ... & Ktistakis, N. T. (2016). Autophagy initiation by ULK complex assembly on ER tubulovesicular regions marked by ATG9 vesicles. Nature communications, 7(1), 12420.
Rabbit Anti-ATG9A Recombinant Antibody (BA0202) (CBMAB-0111CQ) in WB
Western blot analysis of ATG9 expression. ...View More
D'Amico, R., Impellizzeri, D., Cordaro, M., Siracusa, R., Interdonato, L., Marino, Y., ... & Di Paola, R. (2022). Complex Interplay between Autophagy and Oxidative Stress in the Development of Endometriosis. Antioxidants, 11(12), 2484.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
BA0202
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues near the amino terminus of human Atg7 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 9A
Introduction
ATG9A (Autophagy Related 9A) is a Protein Coding gene. Among its related pathways are Cellular Senescence and mTOR signalling. Human ATG9A, which they called APG9L1. The deduced 839-amino acid human protein has an APG9-like domain and predicted transmembrane domains. Northern blot and RT-PCR analysis detected APG9L1 in all tissues examined. Mouse Apg9l1 was also widely expressed. Time-lapse imaging showed that mouse Apg9l1 colocalized with the autophagosome membrane marker Lc3 (MAP1LC3A; 601242) in transfected COS-7 cells. GO annotations related to this gene include heme-copper terminal oxidase activity.
Entrez Gene ID
Human79065
Mouse245860
Rat363254
UniProt ID
HumanQ7Z3C6
MouseQ68FE2
RatQ5FWU3
Alternative Names
Autophagy Related 9A; APG9-Like 1; APG9L1; MATG9; ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae); APG9 Autophagy 9-Like 1 (S. Cerevisiae); ATG9 Autophagy Related 9 Homolog A; Autophagy 9-Like 1 Protein; APG9 Autophagy 9-Like 1; MGD3208
Function
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Biological Process
Autophagosome assembly Source: MGI
Autophagy of mitochondrion Source: GO_Central
Late nucleophagy Source: GO_Central
Protein localization to phagophore assembly site Source: GO_Central
Protein transport Source: UniProtKB-KW
Cellular Location
Late endosome membrane; Trans-Golgi network membrane; Endoplasmic reticulum membrane; Autophagosome membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
Topology
Cytoplasmic: 2-66 aa
Helical: 67-87 aa
Lumenal: 88-131 aa
Helical: 132-152 aa
Cytoplasmic: 153-289 aa
Helical: 290-310 aa
Lumenal: 311-371 aa
Helical: 372-392 aa
Cytoplasmic: 393-400 aa
Helical: 401-421 aa
Lumenal: 422-473 aa
Helical: 474-494 aa
Cytoplasmic: 495-839 aa

Kimkong, I., & Kunanopparat, A. (2020). Autophagy related protein 9A increase in hepatitis B virus-associated hepatocellular carcinoma and the role in apoptosis. World Journal of Hepatology, 12(12), 1367.

Mailler, E., Waheed, A. A., Park, S. Y., Gershlick, D. C., Freed, E. O., & Bonifacino, J. S. (2019). The autophagy protein ATG9A promotes HIV-1 infectivity. Retrovirology, 16(1), 1-20.

Kong, Y., Huang, T., Zhang, H., Zhang, Q., Ren, J., Guo, X., ... & Liu, L. (2019). The lncRNA NEAT1/miR-29b/Atg9a axis regulates IGFBPrP1-induced autophagy and activation of mouse hepatic stellate cells. Life sciences, 237, 116902.

Fan, H., Yuan, F., Yun, Y., Wu, T., Lu, L., Liu, J., ... & Chen, S. Y. (2019). MicroRNA-34a mediates ethanol-induced impairment of neural differentiation of neural crest cells by targeting autophagy-related gene 9a. Experimental neurology, 320, 112981.

Davies, A. K., Itzhak, D. N., Edgar, J. R., Archuleta, T. L., Hirst, J., Jackson, L. P., ... & Borner, G. H. (2018). AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Nature communications, 9(1), 1-21.

Jiang, C. Y., Yang, B. Y., Zhao, S., Shao, S. H., Bei, X. Y., Shi, F., ... & Ruan, Y. (2018). Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression. Cell death & disease, 9(4), 1-12.

Pang, J., Xiong, H., Lin, P., Lai, L., Yang, H., Liu, Y., ... & Zheng, Y. (2017). Activation of miR-34a impairs autophagic flux and promotes cochlear cell death via repressing ATG9A: implications for age-related hearing loss. Cell death & disease, 8(10), e3079-e3079.

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For research use only. Not intended for any clinical use.

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