Mouse Atg9a ELISA Kit (V2LY-0626-LY3313)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.007 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
63.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Autophagy Related 9A
Function
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Biological Process
Autophagosome assembly Source: MGI
Autophagy of mitochondrion Source: GO_Central
Late nucleophagy Source: GO_Central
Protein localization to phagophore assembly site Source: GO_Central
Protein transport Source: UniProtKB-KW
Cellular Location
Late endosome membrane; Trans-Golgi network membrane; Endoplasmic reticulum membrane; Autophagosome membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
Topology
Cytoplasmic: 2-66 aa
Helical: 67-87 aa
Lumenal: 88-131 aa
Helical: 132-152 aa
Cytoplasmic: 153-289 aa
Helical: 290-310 aa
Lumenal: 311-371 aa
Helical: 372-392 aa
Cytoplasmic: 393-400 aa
Helical: 401-421 aa
Lumenal: 422-473 aa
Helical: 474-494 aa
Cytoplasmic: 495-839 aa

Kimkong, I., & Kunanopparat, A. (2020). Autophagy related protein 9A increase in hepatitis B virus-associated hepatocellular carcinoma and the role in apoptosis. World Journal of Hepatology, 12(12), 1367.

Mailler, E., Waheed, A. A., Park, S. Y., Gershlick, D. C., Freed, E. O., & Bonifacino, J. S. (2019). The autophagy protein ATG9A promotes HIV-1 infectivity. Retrovirology, 16(1), 1-20.

Kong, Y., Huang, T., Zhang, H., Zhang, Q., Ren, J., Guo, X., ... & Liu, L. (2019). The lncRNA NEAT1/miR-29b/Atg9a axis regulates IGFBPrP1-induced autophagy and activation of mouse hepatic stellate cells. Life sciences, 237, 116902.

Fan, H., Yuan, F., Yun, Y., Wu, T., Lu, L., Liu, J., ... & Chen, S. Y. (2019). MicroRNA-34a mediates ethanol-induced impairment of neural differentiation of neural crest cells by targeting autophagy-related gene 9a. Experimental neurology, 320, 112981.

Davies, A. K., Itzhak, D. N., Edgar, J. R., Archuleta, T. L., Hirst, J., Jackson, L. P., ... & Borner, G. H. (2018). AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Nature communications, 9(1), 1-21.

Jiang, C. Y., Yang, B. Y., Zhao, S., Shao, S. H., Bei, X. Y., Shi, F., ... & Ruan, Y. (2018). Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression. Cell death & disease, 9(4), 1-12.

Pang, J., Xiong, H., Lin, P., Lai, L., Yang, H., Liu, Y., ... & Zheng, Y. (2017). Activation of miR-34a impairs autophagic flux and promotes cochlear cell death via repressing ATG9A: implications for age-related hearing loss. Cell death & disease, 8(10), e3079-e3079.

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For research use only. Not intended for any clinical use.

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