Mouse Anti-ATP2A1 Recombinant Antibody (1B11) (CBMAB-A4025-YC)

Name: Mouse Anti-ATP2A1 Recombinant Antibody (1B11)
SKU: CBMAB-A4025-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1B11

Application

ELISA, ICC, IF, IHC-P, WB

Immunogen

ATP2A1 (NP_775293, 522 a.a. ~ 612 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 522-613

More Infomation

Target

Full Name

ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1

Introduction

ATP2A1 is one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sar

Entrez Gene ID

487

UniProt ID

O14983

Alternative Names

ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1; Calcium Pump 1; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Fast Twitch Skeletal Muscle Isoform; ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1; Sarcoplasmic/Endoplasmic

Function

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity).
Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).

Biological Process

Apoptotic mitochondrial changes Source: BHF-UCL
Calcium ion import Source: BHF-UCL
Calcium ion import into sarcoplasmic reticulum Source: UniProtKB
Calcium ion transmembrane transport Source: GO_Central
Calcium ion transport Source: UniProtKB
Cellular calcium ion homeostasis Source: GO_Central
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Maintenance of mitochondrion location Source: BHF-UCL
Negative regulation of endoplasmic reticulum calcium ion concentration Source: BHF-UCL
Negative regulation of striated muscle contraction Source: UniProtKB
Positive regulation of ATPase-coupled calcium transmembrane transporter activity Source: UniProtKB
Positive regulation of calcium ion import into sarcoplasmic reticulum Source: UniProtKB
Positive regulation of cardiac muscle cell contraction Source: UniProtKB
Positive regulation of endoplasmic reticulum calcium ion concentration Source: BHF-UCL
Positive regulation of fast-twitch skeletal muscle fiber contraction Source: UniProtKB
Positive regulation of mitochondrial calcium ion concentration Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Regulation of striated muscle contraction Source: UniProtKB
Relaxation of skeletal muscle Source: BHF-UCL
Response to endoplasmic reticulum stress Source: BHF-UCL

Cellular Location

Endoplasmic reticulum membrane; Sarcoplasmic reticulum membrane

Involvement in disease

Brody myopathy (BRM): A disorder of muscle function that is characterized by painless muscle cramping and exercise-induced impairment of muscle relaxation.

Bruels, C. C., Li, C., Mendoza, T., Khan, J., Reddy, H. M., Estrella, E. A., ... & Kang, P. B. (2019). Identification of a pathogenic mutation in ATP2A1 via in silico analysis of exome data for cryptic aberrant splice sites. Molecular genetics & genomic medicine, 7(3), e552.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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