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Mouse Anti-ATP2A1 Recombinant Antibody (VE121G9) (CBMAB-A4027-YC)

Provided herein is a Mouse monoclonal antibody against Human ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1. The antibody can be used for immunoassay techniques, such as ICC, WB.
See all ATP2A1 antibodies
Published Data
Mouse Anti-ATP2A1 Recombinant Antibody (A4027) (CBMAB-A4027-YC) in WB
Western blot analysis of SERCA1, SERCA2, RYR1, SLN, and NNAT. For SERCA2 and RYR1, these targets were probed for on the same membrane and have therefore the same Ponceau. ...View More
Geromella, M. S., Ryan, C. R., Braun, J. L., Finch, M. S., Maddalena, L. A., Bagshaw, O., ... & Fajardo, V. A. (2022). Low-dose lithium supplementation promotes adipose tissue browning and sarco (endo) plasmic reticulum Ca2+ ATPase uncoupling in muscle. Journal of Biological Chemistry, 298(11).
Mouse Anti-ATP2A1 Recombinant Antibody (A4027) (CBMAB-A4027-YC) in IHC
Myopathic changes in paraspinal muscles of camptocormia compared to controls: Increased fiber size spectrum (a H&E) and hypertrophy (g), endomysial fibrosis (c elastica van Gieson stain) and lack of type-2 fibers (e SERCA1 immunohistochemistry) in camptocormia compared to controls (b, d, f). ...View More
Wrede, A., Margraf, N. G., Goebel, H. H., Deuschl, G., & Schulz-Schaeffer, W. J. (2012). Myofibrillar disorganization characterizes myopathy of camptocormia in Parkinson's disease. Acta neuropathologica, 123, 419-432.
Mouse Anti-ATP2A1 Recombinant Antibody (A4027) (CBMAB-A4027-YC) in IF
Co-localisation analysis of CAPN3, with SERCA1, SERCA2 and sAnk1 in longitudinal sections from human dorsal muscle using in situ PLA. Red spots represent protein complexes in close proximity (<40 nm). Sections were double-stained for myosin heavy chain (MF20, green). Neg Ctrl, negative control with just one or no primary antibodies. Scale bar: 20 μm. ...View More
Toral-Ojeda, I., Aldanondo, G., Lasa-Elgarresta, J., Lasa-Fernández, H., Fernández-Torrón, R., de Munain, A. L., & Vallejo-Illarramendi, A. (2016). Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert reviews in molecular medicine, 18, e7.
Mouse Anti-ATP2A1 Recombinant Antibody (A4027) (CBMAB-A4027-YC) in IP
(a) Immunoprecipitation (IP) of CAPN3 with a goat polyclonal antibody (pIS2C) in a vastus lateralis muscle from a healthy donor. Both, SERCA1 and SERCA2 are detected in the CAPN3 IP. White lines indicate noncontiguous lanes run on the same gel. Input: protein extract. (b) SERCA1 and SERCA2 were immunoprecipitated (IP) with specific monoclonal antibodies in the same muscle. sAnk1 is detected in SERCA1 IP but not in SERCA2 IP. ...View More
Toral-Ojeda, I., Aldanondo, G., Lasa-Elgarresta, J., Lasa-Fernández, H., Fernández-Torrón, R., de Munain, A. L., & Vallejo-Illarramendi, A. (2016). Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert reviews in molecular medicine, 18, e7.

Summary

Host Animal
Mouse
Specificity
Amphibian, Dog, Human, Mouse, Pig, Rabbit, Rat, Yeast
Clone
VE121G9
Antibody Isotype
IgG1
Application
ICC, WB, IF, IHC, IP

Basic Information

Immunogen
Purified rabbit skeletal muscle sarcoplasmic reticulum.
Specificity
Amphibian, Dog, Human, Mouse, Pig, Rabbit, Rat, Yeast
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml
IF(ICC)1:500
IHC-P1:20

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1
Introduction
ATP2A1 is one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sar
Entrez Gene ID
487
UniProt ID
O14983
Alternative Names
ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1; Calcium Pump 1; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Fast Twitch Skeletal Muscle Isoform; ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1; Sarcoplasmic/Endoplasmic
Function
Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity).
Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).
Biological Process
Apoptotic mitochondrial changes Source: BHF-UCL
Calcium ion import Source: BHF-UCL
Calcium ion import into sarcoplasmic reticulum Source: UniProtKB
Calcium ion transmembrane transport Source: GO_Central
Calcium ion transport Source: UniProtKB
Cellular calcium ion homeostasis Source: GO_Central
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Maintenance of mitochondrion location Source: BHF-UCL
Negative regulation of endoplasmic reticulum calcium ion concentration Source: BHF-UCL
Negative regulation of striated muscle contraction Source: UniProtKB
Positive regulation of ATPase-coupled calcium transmembrane transporter activity Source: UniProtKB
Positive regulation of calcium ion import into sarcoplasmic reticulum Source: UniProtKB
Positive regulation of cardiac muscle cell contraction Source: UniProtKB
Positive regulation of endoplasmic reticulum calcium ion concentration Source: BHF-UCL
Positive regulation of fast-twitch skeletal muscle fiber contraction Source: UniProtKB
Positive regulation of mitochondrial calcium ion concentration Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Regulation of striated muscle contraction Source: UniProtKB
Relaxation of skeletal muscle Source: BHF-UCL
Response to endoplasmic reticulum stress Source: BHF-UCL
Cellular Location
Endoplasmic reticulum membrane; Sarcoplasmic reticulum membrane
Involvement in disease
Brody myopathy (BRM): A disorder of muscle function that is characterized by painless muscle cramping and exercise-induced impairment of muscle relaxation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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