Mouse Anti-BAG2 Recombinant Antibody (CBYY-1797) (CBMAB-2146-YY)

Name: Mouse Anti-BAG2 Recombinant Antibody (CBYY-1797)
SKU: CBMAB-2146-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BAG2 Recombinant Antibody (CBYY-1797) in IP

Western blot analysis of BAG2 and BAG5 in immunoprecipitated extracts of T47D cells treated with 1 μmol/L X15695 for 48 hours. A total of 70 μg of cell extracts was used as input control while 1,000 μg was immunoprecipitated with anti-p53 or IgG antibodies immobilized on protein A/G agarose beads. ...View More

Pan, M., Solozobova, V., Kuznik, N. C., Jung, N., Gräβle, S., Gourain, V., ... & Cato, A. C. (2023). Identification of an imidazopyridine-based compound as an oral selective estrogen receptor degrader for breast cancer therapy. Cancer Research Communications, 3(7), 1378-1396.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-1797

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-211 representing full length Bag-2 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BCL2-associated athanogene 2

Introduction

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Entrez Gene ID

9532

UniProt ID

O95816

Alternative Names

BCL2 Associated Athanogene 2; Bcl-2-Associated Athanogene 2; BAG-2; DJ417I1.2 (BAG-Family Molecular Chaperone Regulator 2); BAG Family Molecular Chaperone Regulator 2; DJ417I1.2;

Function

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016).

Biological Process

Negative regulation of protein binding Source: ARUK-UCL
Negative regulation of protein ubiquitination Source: ARUK-UCL
Negative regulation of ubiquitin protein ligase activity Source: ARUK-UCL
Positive regulation of proteasomal protein catabolic process Source: ARUK-UCL
Positive regulation of protein processing Source: ARUK-UCL
Protein folding Source: ProtInc
Protein metabolic process Source: MGI
Protein stabilization Source: ARUK-UCL
Regulation of cellular response to heat Source: Reactome

Cellular Location

Dendritic microtubule; Cytosol, Axon; Chaperone complex; Dendrite

Sun, L., Chen, G., Sun, A., Wang, Z., Huang, H., Gao, Z., ... & Li, K. (2020). BAG2 promotes proliferation and metastasis of gastric cancer via ERK1/2 signaling and partially regulated by miR186. Frontiers in oncology, 10, 31.

Schönbühler, B., Schmitt, V., Huesmann, H., Kern, A., Gamerdinger, M., & Behl, C. (2017). BAG2 interferes with CHIP-mediated ubiquitination of HSP72. International journal of molecular sciences, 18(1), 69.

Yang, K. M., Bae, E., Ahn, S. G., Pang, K., Park, Y., Park, J., ... & Kim, S. J. (2017). Co-chaperone BAG2 determines the pro-oncogenic role of cathepsin B in triple-negative breast cancer cells. Cell reports, 21(10), 2952-2964.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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