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Mouse Anti-BAG2 Recombinant Antibody (CBYY-1814) (CBMAB-2163-YY)

This product is mouse antibody that recognizes BAG2. The antibody CBYY-1814 can be used for immunoassay techniques such as: ELISA, WB
See all BAG2 antibodies
Published Data
Mouse Anti-BAG2 Recombinant Antibody (CBYY-1814) in IP
BAG2 antibody was able to detect endogenous protein expression of BAG2. Total cell lysates from SK-N-BE(1) and SH-SY5Y was immunoprecipitated with BAG2 antibody and protein levels were detected with the same BAG2 antibody. ...View More
Shi, Y., Yuan, J., Rraklli, V., Maxymovitz, E., Cipullo, M., Liu, M., ... & Holmberg, J. (2021). Aberrant splicing in neuroblastoma generates RNA-fusion transcripts and provides vulnerability to spliceosome inhibitors. Nucleic acids research, 49(5), 2509-2521.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-1814
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-211 representing full length Bag-2 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BCL2-associated athanogene 2
Introduction
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Entrez Gene ID
9532
UniProt ID
O95816
Alternative Names
BCL2 Associated Athanogene 2; Bcl-2-Associated Athanogene 2; BAG-2; DJ417I1.2 (BAG-Family Molecular Chaperone Regulator 2); BAG Family Molecular Chaperone Regulator 2; DJ417I1.2;
Function
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016).
Biological Process
Negative regulation of protein binding Source: ARUK-UCL
Negative regulation of protein ubiquitination Source: ARUK-UCL
Negative regulation of ubiquitin protein ligase activity Source: ARUK-UCL
Positive regulation of proteasomal protein catabolic process Source: ARUK-UCL
Positive regulation of protein processing Source: ARUK-UCL
Protein folding Source: ProtInc
Protein metabolic process Source: MGI
Protein stabilization Source: ARUK-UCL
Regulation of cellular response to heat Source: Reactome
Cellular Location
Dendritic microtubule; Cytosol, Axon; Chaperone complex; Dendrite
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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