Mouse Anti-BCAM Recombinant Antibody (CBYY-0259) (CBMAB-0260-YY)

Name: Mouse Anti-BCAM Recombinant Antibody (CBYY-0259)
SKU: CBMAB-0260-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BCAM Recombinant Antibody (CBYY-0259) (CBMAB-0260-YY) in Immunoblot

BCAM protein expression in tumor and adjacent normal tissues from 6 patients in West China Hospital cohort by immunoblotting. The normalized data after quantitative analysis were shown on the right. **p<0.01. ...View More

Zhao, J., Liang, J., Yang, Y., Sun, G., Zhang, X., Zhao, J., ... & Zeng, H. (2022). Integrated multi-omics analyses reveal that BCAM is associated with epigenetic modification and tumor microenvironment subtypes of clear cell renal cell carcinoma. Clinical Epigenetics, 14(1), 1-15.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0259

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 258-301 mapping within an internal region of BCAM of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Basal Cell Adhesion Molecule (Lutheran Blood Group)

Introduction

This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. PolymorphisMouse in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforMouse have been found for this gene. [provided by RefSeq, May 2012]

Entrez Gene ID

4059

UniProt ID

P50895

Alternative Names

Basal Cell Adhesion Molecule (Lutheran Blood Group); Basal Cell Adhesion Molecule (Lu And Au Blood Groups); B-CAM Cell Surface Glycoprotein; Auberger B Antigen; F8/G253 Antigen; MouseK19; LU; Lutheran Blood Group (Auberger B Antigen Included); Lutheran Blood Group Variant LUGA;

Function

Laminin alpha-5 receptor. May mediate intracellular signaling.

Biological Process

Cell adhesion Source: ProtInc
Cell-matrix adhesion Source: BHF-UCL
Signal transduction Source: ProtInc

Cellular Location

Membrane

Topology

Extracellular: 32-547 aa
Helical: 548-568 aa
Cytoplasmic: 569-628 aa

PTM

Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.

Wei, H., Cui, Y., Chen, Y., & Mu, S. (2020). The progress of abnormal expression of Lutheran blood group glycoprotein in cancers. Blood and Genomics, 4(1), 39-44.

Guadall, A., Cochet, S., Renaud, O., Colin, Y., Le Van Kim, C., de Brevern, A. G., & El Nemer, W. (2019). Dimerization and phosphorylation of Lutheran/basal cell adhesion molecule are critical for its function in cell migration on laminin. Journal of Biological Chemistry, 294(41), 14911-14921.

Reppin, F., Cochet, S., El Nemer, W., Fritz, G., & Schmidt, G. (2018). High affinity binding of Escherichia coli cytotoxic necrotizing factor 1 (CNF1) to Lu/BCAM adhesion glycoprotein. Toxins, 10(1), 3.

Azuma, M., Tsukada, T., Inagaki, T., Casmad, F., Jindatip, D., Tofrizal, A., ... & Yashiro, T. (2018). Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands. Acta histochemica et cytochemica, 51(5), 145-152.

Klei, T. R. L., de Back, D. Z., Asif, P. J., Verkuijlen, P. J. J. H., Veldthuis, M., Ligthart, P. C., ... & van Bruggen, R. (2018). Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging. Blood advances, 2(1), 14-24.

Miura, Y., Matsui, S., Miyata, N., Harada, K., Kikkawa, Y., Ohmuraya, M., ... & Tanaka, M. (2018). Differential expression of Lutheran/BCAM regulates biliary tissue remodeling in ductular reaction during liver regeneration. Elife, 7, e36572.

Chang, H. Y., Chang, H. M., Wu, T. J., Chaing, C. Y., Tzai, T. S., Cheng, H. L., ... & Liu, H. S. (2017). The role of Lutheran/basal cell adhesion molecule in human bladder carcinogenesis. Journal of biomedical science, 24(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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