Human Recombinant BCAM protein, hFc Tag (V2LY-0526-LY2303)

Name: Human Recombinant BCAM protein, hFc Tag
SKU: V2LY-0526-LY2303
Rating: 5 (1 reviews)

See all BCAM antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant BCAM protein, hFc Tag consist of Amino Acid: 1-547 and predicts a molecular mass of 83 kDa.

Molecule Mass

83 kDa

Verified

HPLC

Sequence

Amino Acid: 1-547

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Basal Cell Adhesion Molecule (Lutheran Blood Group)

Function

Laminin alpha-5 receptor. May mediate intracellular signaling.

Biological Process

Cell adhesion Source: ProtInc
Cell-matrix adhesion Source: BHF-UCL
Signal transduction Source: ProtInc

Cellular Location

Membrane

Topology

Extracellular: 32-547 aa
Helical: 548-568 aa
Cytoplasmic: 569-628 aa

PTM

Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.

Wei, H., Cui, Y., Chen, Y., & Mu, S. (2020). The progress of abnormal expression of Lutheran blood group glycoprotein in cancers. Blood and Genomics, 4(1), 39-44.

Guadall, A., Cochet, S., Renaud, O., Colin, Y., Le Van Kim, C., de Brevern, A. G., & El Nemer, W. (2019). Dimerization and phosphorylation of Lutheran/basal cell adhesion molecule are critical for its function in cell migration on laminin. Journal of Biological Chemistry, 294(41), 14911-14921.

Reppin, F., Cochet, S., El Nemer, W., Fritz, G., & Schmidt, G. (2018). High affinity binding of Escherichia coli cytotoxic necrotizing factor 1 (CNF1) to Lu/BCAM adhesion glycoprotein. Toxins, 10(1), 3.

Azuma, M., Tsukada, T., Inagaki, T., Casmad, F., Jindatip, D., Tofrizal, A., ... & Yashiro, T. (2018). Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands. Acta histochemica et cytochemica, 51(5), 145-152.

Klei, T. R. L., de Back, D. Z., Asif, P. J., Verkuijlen, P. J. J. H., Veldthuis, M., Ligthart, P. C., ... & van Bruggen, R. (2018). Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging. Blood advances, 2(1), 14-24.

Miura, Y., Matsui, S., Miyata, N., Harada, K., Kikkawa, Y., Ohmuraya, M., ... & Tanaka, M. (2018). Differential expression of Lutheran/BCAM regulates biliary tissue remodeling in ductular reaction during liver regeneration. Elife, 7, e36572.

Chang, H. Y., Chang, H. M., Wu, T. J., Chaing, C. Y., Tzai, T. S., Cheng, H. L., ... & Liu, H. S. (2017). The role of Lutheran/basal cell adhesion molecule in human bladder carcinogenesis. Journal of biomedical science, 24(1), 1-14.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant BCAM protein, hFc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BCAM protein, His Tag (CAT#: V2LY-0526-LY2304)

Human Recombinant BCAM protein, His Tag-1 (CAT#: V2LY-0526-LY2305)

Mouse Recombinant BCAM protein, hFc Tag (CAT#: V2LY-0526-LY7860)

Mouse Recombinant BCAM protein, His Tag (CAT#: V2LY-0526-LY7861)

Rat Recombinant BCAM protein, His Tag (CAT#: V2LY-0526-LY9180)

Related Products

Mouse Anti-BCAM Recombinant Antibody (CBYY-0267) (CAT#: CBMAB-0268-YY)

Mouse Anti-BCAM Recombinant Antibody (CBFYC-0574) (CAT#: CBMAB-C0628-FY)

Mouse Anti-BCAM Recombinant Antibody (CBT4466) (CAT#: V2LY-0625-LY1217)

Mouse Anti-BCAM Recombinant Antibody (CBYY-0269) (CAT#: CBMAB-0270-YY)

Mouse Recombinant BCAM protein, His Tag (CAT#: V2LY-0526-LY7861)

Human Recombinant BCAM protein, His Tag (CAT#: V2LY-0526-LY2304)

Rat Recombinant BCAM protein, His Tag (CAT#: V2LY-0526-LY9180)

Mouse Anti-BCAM Recombinant Antibody (CBYY-1707) (CAT#: CBMAB-2056-YY)

Mouse Anti-BCAM Recombinant Antibody (CBT2201) (CAT#: V2LY-0625-LY1216)

Rabbit Anti-BCAM Recombinant Antibody (CBYY-C0767) (CAT#: CBMAB-C2203-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry