Human Recombinant BCAM protein, His Tag (V2LY-0526-LY2304)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant BCAM protein, His Tag consist of Amino Acid: 1-547 and predicts a molecular mass of 57.7 kDa.
Molecule Mass
57.7 kDa
Sequence
Amino Acid: 1-547
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Endotoxin
<0.5 EU/mg protein.
Format
Liquid
Buffer
Tris, NaCl, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Basal Cell Adhesion Molecule (Lutheran Blood Group)
Function
Laminin alpha-5 receptor. May mediate intracellular signaling.
Biological Process
Cell adhesion Source: ProtInc
Cell-matrix adhesion Source: BHF-UCL
Signal transduction Source: ProtInc
Cellular Location
Membrane
Topology
Extracellular: 32-547 aa
Helical: 548-568 aa
Cytoplasmic: 569-628 aa
PTM
Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.

Wei, H., Cui, Y., Chen, Y., & Mu, S. (2020). The progress of abnormal expression of Lutheran blood group glycoprotein in cancers. Blood and Genomics, 4(1), 39-44.

Guadall, A., Cochet, S., Renaud, O., Colin, Y., Le Van Kim, C., de Brevern, A. G., & El Nemer, W. (2019). Dimerization and phosphorylation of Lutheran/basal cell adhesion molecule are critical for its function in cell migration on laminin. Journal of Biological Chemistry, 294(41), 14911-14921.

Reppin, F., Cochet, S., El Nemer, W., Fritz, G., & Schmidt, G. (2018). High affinity binding of Escherichia coli cytotoxic necrotizing factor 1 (CNF1) to Lu/BCAM adhesion glycoprotein. Toxins, 10(1), 3.

Azuma, M., Tsukada, T., Inagaki, T., Casmad, F., Jindatip, D., Tofrizal, A., ... & Yashiro, T. (2018). Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands. Acta histochemica et cytochemica, 51(5), 145-152.

Klei, T. R. L., de Back, D. Z., Asif, P. J., Verkuijlen, P. J. J. H., Veldthuis, M., Ligthart, P. C., ... & van Bruggen, R. (2018). Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging. Blood advances, 2(1), 14-24.

Miura, Y., Matsui, S., Miyata, N., Harada, K., Kikkawa, Y., Ohmuraya, M., ... & Tanaka, M. (2018). Differential expression of Lutheran/BCAM regulates biliary tissue remodeling in ductular reaction during liver regeneration. Elife, 7, e36572.

Chang, H. Y., Chang, H. M., Wu, T. J., Chaing, C. Y., Tzai, T. S., Cheng, H. L., ... & Liu, H. S. (2017). The role of Lutheran/basal cell adhesion molecule in human bladder carcinogenesis. Journal of biomedical science, 24(1), 1-14.

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For research use only. Not intended for any clinical use.

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