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Mouse Anti-BCAR1 Recombinant Antibody (1B1) (CBMAB-P0091-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
1B1
Antibody Isotype
IgG1
Application
WB, FC, IHC, ICC, IF

Basic Information

Immunogen
Full length human recombinant protein of human BCAR1 produced in HEK293T cell.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
FC1:100
IF(ICC)1:100
IHC1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Introduction
BCAR1 is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.
Entrez Gene ID
Human9564
Mouse12927
Rat25414
Chicken415691
Dog479648
UniProt ID
HumanP56945
MouseQ61140
RatQ63767
ChickenA0A1D5P1L0
DogF1P9X8
Alternative Names
BCAR1, Cas Family Scaffolding Protein; Cas Scaffolding Protein Family Member 1; Crk-Associated Substrate; P130Cas; CASS1; CRKAS;
Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed:12832404, PubMed:12432078).
Implicated in induction of cell migration and cell branching (PubMed:12432078, PubMed:12832404, PubMed:17038317).
Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).
Biological Process
Actin filament organization Source: UniProtKB
Actin filament reorganization Source: GO_Central
Antigen receptor-mediated signaling pathway Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Cell chemotaxis Source: BHF-UCL
Cell division Source: UniProtKB
Cell migration Source: UniProtKB
Cellular response to hepatocyte growth factor stimulus Source: BHF-UCL
Endothelin receptor signaling pathway Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Hepatocyte growth factor receptor signaling pathway Source: BHF-UCL
Insulin receptor signaling pathway Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of endothelial cell migration Source: BHF-UCL
Regulation of apoptotic process Source: UniProtKB
Regulation of cell growth Source: UniProtKB
T cell receptor signaling pathway Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
Cellular Location
Cytoplasm; Focal adhesion; Axon. Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity).
PTM
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128.
Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner; phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.
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For research use only. Not intended for any clinical use.

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