Mouse Anti-BCAR1 Recombinant Antibody (CBYY-0286) (CBMAB-0287-YY)

Name: Mouse Anti-BCAR1 Recombinant Antibody (CBYY-0286)
SKU: CBMAB-0287-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0286

Application

Immunogen

E. coli-derived recombinant human p130Cas Met66-Val412.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BCAR1, Cas Family Scaffolding Protein

Introduction

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]

Entrez Gene ID

9564

UniProt ID

P56945

Alternative Names

BCAR1, Cas Family Scaffolding Protein; Cas Scaffolding Protein Family Member 1; Crk-Associated Substrate; P130Cas; CASS1; CRKAS;

Function

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed:12832404, PubMed:12432078).
Implicated in induction of cell migration and cell branching (PubMed:12432078, PubMed:12832404, PubMed:17038317).
Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).

Biological Process

Actin filament organization Source: UniProtKB
Actin filament reorganization Source: GO_Central
Antigen receptor-mediated signaling pathway Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Cell chemotaxis Source: BHF-UCL
Cell division Source: UniProtKB
Cell migration Source: UniProtKB
Cellular response to hepatocyte growth factor stimulus Source: BHF-UCL
Endothelin receptor signaling pathway Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Hepatocyte growth factor receptor signaling pathway Source: BHF-UCL
Insulin receptor signaling pathway Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of endothelial cell migration Source: BHF-UCL
Regulation of apoptotic process Source: UniProtKB
Regulation of cell growth Source: UniProtKB
T cell receptor signaling pathway Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL

Cellular Location

Cytoplasm; Focal adhesion; Axon. Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity).

PTM

PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128.
Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner; phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.

Bauer, J., Gombocz, E., Schulz, H., Hauslage, J., & Grimm, D. (2021). Interaction Network Provides Clues on the Role of Bcar1 in Cellular Response to Changes in Gravity. Computation, 9(8), 81.

Guo, A. K., Itahana, Y., Seshachalam, V. P., Chow, H. Y., Ghosh, S., & Itahana, K. (2021). Mutant TP53 interacts with BCAR1 to contribute to cancer cell invasion. British Journal of Cancer, 124(1), 299-312.

Wisniewski, L., French, V., Lockwood, N., Valdivia, L. E., & Frankel, P. (2020). P130Cas/bcar1 mediates zebrafish caudal vein plexus angiogenesis. Scientific reports, 10(1), 1-14.

Kuikui, Z., Yangchao, Z., Lu, W., Sijia, Z., Yan, Z., Zhenyu, L., ... & Meng, R. (2020). Novel Germline Mutation in BCAR1 in Two Siblings With Lung Cancer: a Possible Susceptibility Gene.

Jiang, S., Mao, C., Jiang, B., Tan, Q., & Deng, B. (2020). High Expression of BCAR1 by Circulating Tumor Cells and Tumor Tissues Is Predictive of a Poor Prognosis of Early-Stage Lung Adenocarcinoma Potentially Due to Regulation of Epithelial-Mesenchymal Transition. Technology in cancer research & treatment, 19, 1533033820983086.

Li, Y., Luo, K., Yin, Y., Wu, C., Deng, M., Li, L., ... & Yuan, J. (2017). USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response. Nature communications, 8(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

BCAR1 Matched Antibody Pair (132) (CAT#: APMAB-132LY)

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BCAR1 Matched Antibody Pair (132) (CAT#: APMAB-132LY)

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Isotype control

For research use only. Not intended for any clinical use.

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