Human BCAR1 ELISA Kit (V2LY-0626-LY3473)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-6 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
93.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
BCAR1, Cas Family Scaffolding Protein
Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed:12832404, PubMed:12432078).
Implicated in induction of cell migration and cell branching (PubMed:12432078, PubMed:12832404, PubMed:17038317).
Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).
Biological Process
Actin filament organization Source: UniProtKB
Actin filament reorganization Source: GO_Central
Antigen receptor-mediated signaling pathway Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Cell chemotaxis Source: BHF-UCL
Cell division Source: UniProtKB
Cell migration Source: UniProtKB
Cellular response to hepatocyte growth factor stimulus Source: BHF-UCL
Endothelin receptor signaling pathway Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Hepatocyte growth factor receptor signaling pathway Source: BHF-UCL
Insulin receptor signaling pathway Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of endothelial cell migration Source: BHF-UCL
Regulation of apoptotic process Source: UniProtKB
Regulation of cell growth Source: UniProtKB
T cell receptor signaling pathway Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
Cellular Location
Cytoplasm; Focal adhesion; Axon. Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity).
PTM
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128.
Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner; phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.

Bauer, J., Gombocz, E., Schulz, H., Hauslage, J., & Grimm, D. (2021). Interaction Network Provides Clues on the Role of Bcar1 in Cellular Response to Changes in Gravity. Computation, 9(8), 81.

Guo, A. K., Itahana, Y., Seshachalam, V. P., Chow, H. Y., Ghosh, S., & Itahana, K. (2021). Mutant TP53 interacts with BCAR1 to contribute to cancer cell invasion. British Journal of Cancer, 124(1), 299-312.

Wisniewski, L., French, V., Lockwood, N., Valdivia, L. E., & Frankel, P. (2020). P130Cas/bcar1 mediates zebrafish caudal vein plexus angiogenesis. Scientific reports, 10(1), 1-14.

Kuikui, Z., Yangchao, Z., Lu, W., Sijia, Z., Yan, Z., Zhenyu, L., ... & Meng, R. (2020). Novel Germline Mutation in BCAR1 in Two Siblings With Lung Cancer: a Possible Susceptibility Gene.

Jiang, S., Mao, C., Jiang, B., Tan, Q., & Deng, B. (2020). High Expression of BCAR1 by Circulating Tumor Cells and Tumor Tissues Is Predictive of a Poor Prognosis of Early-Stage Lung Adenocarcinoma Potentially Due to Regulation of Epithelial-Mesenchymal Transition. Technology in cancer research & treatment, 19, 1533033820983086.

Li, Y., Luo, K., Yin, Y., Wu, C., Deng, M., Li, L., ... & Yuan, J. (2017). USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response. Nature communications, 8(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

BCAR1 Matched Antibody Pair (132) (CAT#: APMAB-132LY)

Antibody Pairs

BCAR1 Matched Antibody Pair (132) (CAT#: APMAB-132LY)

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BCAR1 Matched Antibody Pair (132) (CAT#: APMAB-132LY)

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Isotype control

For research use only. Not intended for any clinical use.

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