Mouse Anti-BDKRB1 Recombinant Antibody (EG383) (CBMAB-EN424-LY)

Name: Mouse Anti-BDKRB1 Recombinant Antibody (EG383)
SKU: CBMAB-EN424-LY
Rating: 5 (1 reviews)

See all BDKRB1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG383

Application

WB, IHC, IF, IP

Immunogen

Amino acids 230-250 of bradykinin B1 R of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Bradykinin Receptor B1

Introduction

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Entrez Gene ID

623

UniProt ID

P46663

Alternative Names

Bradykinin Receptor B1; BK-1 Receptor; BRADYB1; B1R; B1 Bradykinin Receptor; Bradykinin B1 Receptor; Bradykinin Receptor B2;

Function

This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
GO - Molecular functioni

Biological Process

Cell migration Source: Ensembl
G protein-coupled receptor signaling pathway Source: GO_Central
Inflammatory response Source: Ensembl
Negative regulation of blood pressure Source: Ensembl
Negative regulation of cell growth Source: Ensembl
Negative regulation of protein phosphorylation Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: ProtInc
Positive regulation of leukocyte migration Source: Ensembl
Positive regulation of release of sequestered calcium ion into cytosol Source: Ensembl
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Response to mechanical stimulus Source: InterPro
Sensory perception of pain Source: Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 1-40 aa
Helical: 41-64 aa
Cytoplasmic: 65-73 aa
Helical: 74-98 aa
Extracellular: 99-111 aa
Helical: 112-133 aa
Cytoplasmic: 134-155 aa
Helical: 156-178 aa
Extracellular: 179-199 aa
Helical: 200-226 aa
Cytoplasmic: 227-247 aa
Helical: 248-272 aa
Extracellular: 273-291 aa
Helical: 292-314 aa
Cytoplasmic: 315-353 aa

Xie, H., Lu, F., Liu, W., Wang, E., Wang, L., & Zhong, M. (2021). Remimazolam alleviates neuropathic pain via regulating bradykinin receptor B1 and autophagy. Journal of Pharmacy and Pharmacology.

Sun, D. P., Lee, Y. W., Chen, J. T., Lin, Y. W., & Chen, R. M. (2020). The bradykinin-BDKRB1 axis regulates aquaporin 4 gene expression and consequential migration and invasion of malignant glioblastoma cells via a Ca2+-MEK1-ERK1/2-NF-κB Mechanism. Cancers, 12(3), 667.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-BDKRB1 Recombinant Antibody (EG383)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

BDKRB1 Matched Antibody Pair (135) (CAT#: APMAB-135LY)

Related Products

Human BDKRB1 ELISA Kit (CAT#: V2LY-0626-LY5498)

BDKRB1 Matched Antibody Pair (135) (CAT#: APMAB-135LY)

Mouse Anti-BDKRB1 Recombinant Antibody (CBYY-2018) (CAT#: CBMAB-2367-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry