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Mouse Anti-BGLAP Recombinant Antibody (E6) (CBMAB-O0156-CQ)

This product is a mouse antibody that recognizes BGLAP. The antibody OCG4 can be used for immunoassay techniques such as: ELISA, IHC-Fr, IHC-P, WB.
See all BGLAP antibodies
Published Data
Fig.1 Representative images of IHC staining of OCN in CG at weeks 1 post-STZ induction.
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Fig.2 Characterization of the BMSC line by immunocytochemical analysis of the following markers: osteocalcin.
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Fig.3 Representative Western blot images demonstrated that protein expression of Runx-7, SP7 and OCN in PDLSCs was measured by Western blotting after osteogenic induction with 10 and 20 ng/ml of IL-7 for 2 weeks of culture.
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Summary

Host Animal
Mouse
Specificity
Mouse
Clone
E6
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IHC, WB

Basic Information

Immunogen
Amino acids 41-79 within an internal region of osteocalcin of mouse origin.
Host Species
Mouse
Specificity
Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Bone Gamma-Carboxyglutamate Protein
Introduction
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
Entrez Gene ID
Human632
Cattle281646
Pig397530
Rabbit100340503
Dog403762
Goat100861188
Sheep677670
UniProt ID
HumanP02818
CattleP02820
PigQ8HYY9
RabbitP39056
DogP81455
GoatP0C225
SheepW5P6H4
Alternative Names
Bone Gamma-Carboxyglutamate (Gla) Protein; Gamma-Carboxyglutamic Acid-Containing Protein; BGP; Bone Gamma-Carboxyglutamate (Gla) Protein (Osteocalcin); Bone Gla Protein; Osteocalcin; OCN
Function
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Biological Process
Bone development Source: GO_Central
Bone mineralization Source: UniProtKB
Cell adhesion Source: UniProtKB
Cell aging Source: Ensembl
Cellular response to growth factor stimulus Source: Ensembl
Cellular response to vitamin D Source: Ensembl
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Odontogenesis Source: Ensembl
Osteoblast development Source: Ensembl
Osteoblast differentiation Source: BHF-UCL
Regulation of bone mineralization Source: InterPro
Regulation of bone resorption Source: UniProtKB
Regulation of cellular response to insulin stimulus Source: InterPro
Regulation of osteoclast differentiation Source: UniProtKB
Response to activity Source: Ensembl
Response to drug Source: Ensembl
Response to estrogen Source: Ensembl
Response to ethanol Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to gravity Source: Ensembl
Response to hydroxyisoflavone Source: Ensembl
Response to mechanical stimulus Source: Ensembl
Response to testosterone Source: Ensembl
Response to vitamin D Source: BHF-UCL
Response to vitamin K Source: Ensembl
Response to zinc ion Source: Ensembl
Skeletal system development Source: ProtInc
Cellular Location
Secreted
PTM
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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