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Mouse Anti-BLVRA Recombinant Antibody (F1) (CBMAB-A0801-LY)

The product is antibody recognizes BLVRA. The antibody 4G4-2B6 immunoassay techniques such as: WB, ELISA.
See all BLVRA antibodies
Published Data
CBMAB-A0801-LY-1
CBMAB-A0801-LY in WB
The representative bands of BVRA protein were shown by western blot analysis and semiquantified with GAPDH as reference. ...View More
Huang, Y., Liu, Y., Yu, S., Li, W., Li, J., Zhao, B., ... & Jin, H. (2022). Biliverdin reductase A protects lens epithelial cells against oxidative damage and cellular senescence in age-related cataract. Oxidative Medicine and Cellular Longevity, 2022.
CBMAB-A0801-LY-2
CBMAB-A0801-LY in IF
The young and senescent LECs were exposed to 200 μM H2O2 for 4 h. Cells were immunostained with antibody against BVRA (red) and DAPI (blue). ...View More
Huang, Y., Liu, Y., Yu, S., Li, W., Li, J., Zhao, B., ... & Jin, H. (2022). Biliverdin reductase A protects lens epithelial cells against oxidative damage and cellular senescence in age-related cataract. Oxidative Medicine and Cellular Longevity, 2022.
CBMAB-A0801-LY-3
CBMAB-A0801-LY in WB
After silencing BVRA expression, LECs were pretreated with BV/BR (20 μM) for 2 h or damaged with H2O2 (200 μM) for 24 h. The representative bands of cleaved caspase-3 were shown by western blotting analysis and semi-quantified with GAPDH as reference. ...View More
Huang, Y., Li, J., Li, W., Ai, N., & Jin, H. (2022). Biliverdin/bilirubin redox pair protects lens epithelial cells against oxidative stress in age-related cataract by regulating NF-κB/iNOS and Nrf2/HO-1 pathways. Oxidative medicine and cellular longevity, 2022.
CBMAB-A0801-LY-4
CBMAB-A0801-LY in WB
(A) Representative immunoblots illustrating the common affected proteins in the hippocampus after surgery. (B) Sevoflurane anesthesia plus surgery reduced 14-3-3β/α and increased haptoglobin, S100A6, and biliverdin reductase-A expression levels in the hippocampus. ...View More
Li, Y., Chen, L., Li, Z., Song, Y., Yuan, Y., Liu, T., ... & Guo, X. (2021). Potential serum biomarkers for postoperative neurocognitive disorders based on proteomic analysis of cognitive-related brain regions. Frontiers in Aging Neuroscience, 13, 741263.
CBMAB-A0801-LY-5
CBMAB-A0801-LY in IP
mTORC2 is a regulator of BVRA intermolecular interactions. (a) Healthy donor's blood was untreated (lane 1) or treated with biliverdin (50 μM; lane 2-6) for the indicated times. Leukocytes were isolated, lysed, normalized for protein content and analyzed by western blotting. (b) Healthy donor's blood was untreated (UN; lane 1) or treated for 1 with biliverdin alone (50 μM; lane 2), biliverdin plus torin (50 nM; lane 3), plus PKCζ inhibitory peptide (PKCζ-P) (10 μM; lane 4), or plus GSK2334470 (GSK, 3 μM; lane 5). Leukocytes were isolated and lysed. Lysate containing equal total protein amounts (Total) were analyzed by western blotting or were subjected to BVRA-immunoprecipitation (IP). BVRA co-immunoprecipitated proteins were detected by western blotting. ...View More
Zhang, Z., Amorosa, L. F., Petrova, A., Coyle, S., Macor, M., Nair, M., ... & Haimovich, B. (2019). TLR4 counteracts BVRA signaling in human leukocytes via differential regulation of AMPK, mTORC1 and mTORC2. Scientific reports, 9(1), 7020.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
F1
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 75-296 mapping at the C-terminus of BLVRA of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
biliverdin reductase A
Introduction
Biliverdin reductases, such as BLVRA (EC 1.3.1.24), catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH (Komuro et al., 1996 [PubMed 8950184]).[supplied by OMIM
Entrez Gene ID
644
UniProt ID
P53004
Alternative Names
BLVR; BVR; BVRA
Function
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
Biological Process
Heme catabolic process Source: UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Hyperbiliverdinemia (HBLVD): A condition characterized by a green discoloration of the skin, urine, serum, and other bodily fluids. It is due to increased biliverdin resulting from inefficient conversion to bilirubin. Affected individuals appear to have symptoms only in the context of obstructive cholestasis and/or liver failure. In some cases, green jaundice can resolve after resolution of obstructive cholestasis.

Stec, D. E., Gordon, D. M., Nestor-Kalinoski, A. L., Donald, M. C., Mitchell, Z. L., Creeden, J. F., & Hinds, T. D. (2020). Biliverdin reductase A (BVRA) knockout in adipocytes induces hypertrophy and reduces mitochondria in white fat of obese mice. Biomolecules, 10(3), 387.

Kim, S. J., Shin, M. J., Kim, D. W., Yeo, H. J., Yeo, E. J., Choi, Y. J., ... & Choi, S. Y. (2020). Tat-Biliverdin Reductase A Exerts a Protective Role in Oxidative Stress-Induced Hippocampal Neuronal Cell Damage by Regulating the Apoptosis and MAPK Signaling. International journal of molecular sciences, 21(8), 2672.

Mao, H., Xu, Y., Zhang, Z., Sun, G., Wang, Z., Qiao, D., ... & Bo, P. (2020). Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway. Cancer Management and Research, 12, 2697.

Kobachi, K., Kuno, S., Sato, S., Sumiyama, K., Matsuda, M., & Terai, K. (2020). Biliverdin reductase-A deficiency brighten and sensitize biliverdin-binding chromoproteins. Cell Structure and Function, 20010.

Gordon, D. M., Adeosun, S. O., Ngwudike, S. I., Anderson, C. D., Hall, J. E., Hinds Jr, T. D., & Stec, D. E. (2019). CRISPR Cas9-mediated deletion of biliverdin reductase A (BVRA) in mouse liver cells induces oxidative stress and lipid accumulation. Archives of biochemistry and biophysics, 672, 108072.

Zhang, Y., Ding, Y., Lu, T., Zhang, Y., Xu, N., McBride, D. W., ... & Zhang, J. H. (2018). Biliverdin reductase-A attenuated GMH-induced inflammatory response in the spleen by inhibiting toll-like receptor-4 through eNOS/NO pathway. Journal of neuroinflammation, 15(1), 1-12.

Adeosun, S. O., Gordon, D. M., Weeks, M. F., Moore, K. H., Hall, J. E., Hinds Jr, T. D., & Stec, D. E. (2018). Loss of biliverdin reductase-A promotes lipid accumulation and lipotoxicity in mouse proximal tubule cells. American Journal of Physiology-Renal Physiology, 315(2), F323-F331.

Lee, S. J., Kang, H. K., Eum, W. S., Park, J., Choi, S. Y., & Kwon, H. Y. (2017). Tat‐biliverdin reductase A protects INS‐1 cells from human islet amyloid polypeptide‐induced cytotoxicity by alleviating oxidative stress and ER stress. Cell biology international, 41(5), 514-524.

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For research use only. Not intended for any clinical use.

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