Human BLVRA ELISA Kit (V2LY-0626-LY3471)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.023 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
33.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
biliverdin reductase A
Function
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
Biological Process
Heme catabolic process Source: UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Hyperbiliverdinemia (HBLVD): A condition characterized by a green discoloration of the skin, urine, serum, and other bodily fluids. It is due to increased biliverdin resulting from inefficient conversion to bilirubin. Affected individuals appear to have symptoms only in the context of obstructive cholestasis and/or liver failure. In some cases, green jaundice can resolve after resolution of obstructive cholestasis.

Stec, D. E., Gordon, D. M., Nestor-Kalinoski, A. L., Donald, M. C., Mitchell, Z. L., Creeden, J. F., & Hinds, T. D. (2020). Biliverdin reductase A (BVRA) knockout in adipocytes induces hypertrophy and reduces mitochondria in white fat of obese mice. Biomolecules, 10(3), 387.

Kim, S. J., Shin, M. J., Kim, D. W., Yeo, H. J., Yeo, E. J., Choi, Y. J., ... & Choi, S. Y. (2020). Tat-Biliverdin Reductase A Exerts a Protective Role in Oxidative Stress-Induced Hippocampal Neuronal Cell Damage by Regulating the Apoptosis and MAPK Signaling. International journal of molecular sciences, 21(8), 2672.

Mao, H., Xu, Y., Zhang, Z., Sun, G., Wang, Z., Qiao, D., ... & Bo, P. (2020). Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway. Cancer Management and Research, 12, 2697.

Kobachi, K., Kuno, S., Sato, S., Sumiyama, K., Matsuda, M., & Terai, K. (2020). Biliverdin reductase-A deficiency brighten and sensitize biliverdin-binding chromoproteins. Cell Structure and Function, 20010.

Gordon, D. M., Adeosun, S. O., Ngwudike, S. I., Anderson, C. D., Hall, J. E., Hinds Jr, T. D., & Stec, D. E. (2019). CRISPR Cas9-mediated deletion of biliverdin reductase A (BVRA) in mouse liver cells induces oxidative stress and lipid accumulation. Archives of biochemistry and biophysics, 672, 108072.

Zhang, Y., Ding, Y., Lu, T., Zhang, Y., Xu, N., McBride, D. W., ... & Zhang, J. H. (2018). Biliverdin reductase-A attenuated GMH-induced inflammatory response in the spleen by inhibiting toll-like receptor-4 through eNOS/NO pathway. Journal of neuroinflammation, 15(1), 1-12.

Adeosun, S. O., Gordon, D. M., Weeks, M. F., Moore, K. H., Hall, J. E., Hinds Jr, T. D., & Stec, D. E. (2018). Loss of biliverdin reductase-A promotes lipid accumulation and lipotoxicity in mouse proximal tubule cells. American Journal of Physiology-Renal Physiology, 315(2), F323-F331.

Lee, S. J., Kang, H. K., Eum, W. S., Park, J., Choi, S. Y., & Kwon, H. Y. (2017). Tat‐biliverdin reductase A protects INS‐1 cells from human islet amyloid polypeptide‐induced cytotoxicity by alleviating oxidative stress and ER stress. Cell biology international, 41(5), 514-524.

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For research use only. Not intended for any clinical use.

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