Mouse Anti-BNIP1 Recombinant Antibody (CBYY-0692) (CBMAB-0695-YY)

Name: Mouse Anti-BNIP1 Recombinant Antibody (CBYY-0692)
SKU: CBMAB-0695-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0692

Application

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-199 of human BNIP1 (NP_001196) produced in E.coli.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

C-Terminus

More Infomation

Target

Full Name

BCL2/adenovirus E1B 19kDa interacting protein 1

Introduction

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Entrez Gene ID

662

UniProt ID

Q12981

Alternative Names

BCL2 Interacting Protein 1; BCL2/Adenovirus E1B 19 KDa Protein-Interacting Protein 1; BCL2/Adenovirus E1B 19kDa Interacting Protein 1; Transformation-Related Gene 8 Protein; TRG-8; NIP1; Vesicle Transport Protein SEC20; SEC20L; SEC20;

Function

As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization (PubMed:15272311).
Plays also a role in apoptosis (PubMed:7954800, PubMed:15272311, PubMed:23896122).
It is for instance required for endoplasmic reticulum stress-induced apoptosis (PubMed:23896122).
As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).

Biological Process

Apoptotic process Source: MGI
Endoplasmic reticulum membrane fusion Source: HGNC-UCL
Endoplasmic reticulum organization Source: HGNC-UCL
Execution phase of apoptosis Source: BHF-UCL
Negative regulation of apoptotic process Source: UniProtKB
Response to activity Source: Ensembl
Response to oxygen-glucose deprivation Source: Ensembl
Response to starvation Source: Ensembl
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central
Viral process Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane; Mitochondrion membrane. Localization to the mitochondrion is regulated by RNF186.

Topology

Cytoplasmic: 1-199 aa
Helical: 200-220 aa
Lumenal: 221-228 aa

PTM

Polyubiquitinated (PubMed:21931693, PubMed:23896122). 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1 (PubMed:21931693). Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion (PubMed:23896122).

Handschuh, L., Wojciechowski, P., Kazmierczak, M., & Lewandowski, K. (2021). Transcript-Level Dysregulation of BCL2 Family Genes in Acute Myeloblastic Leukemia. Cancers, 13(13), 3175.

Zhou, Z., Peng, X., Tao, X., Zhou, P., Zhang, K., Peng, J., ... & Yang, L. (2021). EBV-LMP1 promotes radioresistance by inducing protective autophagy through BNIP3 in nasopharyngeal carcinoma. Cell death & disease, 12(4), 1-12.

Nishiwaki, Y., & Masai, I. (2020). β-SNAP activity in the outer segment growth period is critical for preventing BNip1-dependent apoptosis in zebrafish photoreceptors. Scientific reports, 10(1), 1-17.

Park, J., Han, J. H., Myung, S. H., Seo, Y. W., & Kim, T. H. (2018). MTD-like motif of a BH3-only protein, BNIP1, induces necrosis accompanied by an intracellular calcium spike. Biochemical and biophysical research communications, 495(2), 1661-1667.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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